Point-of-Care Amperometric Testing of Diabetic Marker ( HbA1c) Using Specific Electroactive Antibodies

被引：19

作者：

Chopra, Adity ^{[1
]}

Rawat, Sanjay ^{[1
]}

Bhalla, Vijayender ^{[1
]}

Suri, C. Raman ^{[1
]}

机构：

[1] Inst Microbial Technol CSIR, Sect 39A, Chandigarh 160036, India

来源：

ELECTROANALYSIS | 2014年 / 26卷 / 03期

关键词：

HbA1c; Ferrocene tagged antibody; Self-assembled monolayer; Electrochemical immunoassay; IMMUNOASSAY; PERFORMANCE; HBA(1C);

D O I：

10.1002/elan.201300646

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

Specific immune detection of glycated hemoglobin is still a great challenge owing to the small epitopic difference between Hemoglobin (Hb) and HbA1c. We report a new electrochemical immunoassay format for point of care testing of HbA1c. A conducting self-assembled monolayer of mercaptophenyl boronic acid (MPBA) was used as a capture layer for binding of glycated proteins and ferrocene tagged anti-HbA1c antibody (FcAb) as a tracer molecule on a gold screen printed electrode. Validation of the new HbA1c assay was carried out using 6 clinical samples with known HPLC values and a correlation coefficient of 98% was observed.

引用

页码：469 / 472

页数：4

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