Effect of ration size on fillet fatty acid composition, phospholipid allostasis and mRNA expression patterns of lipid regulatory genes in gilthead sea bream (Sparus aurata)

被引:41
|
作者
Benedito-Palos, Laura [1 ]
Calduch-Giner, Josep A. [1 ]
Ballester-Lozano, Gabriel F. [1 ]
Perez-Sanchez, Jaume [1 ]
机构
[1] IATS CSIC, Inst Acuicultura Torre Sal, Castellon de La Plana, Spain
关键词
Muscle; Lipid metabolism; Lipoprotein lipase; Stearoyl-CoA desaturase; COD GADUS-MORHUA; TROUT ONCORHYNCHUS-MYKISS; TUMOR-NECROSIS-FACTOR; ACYL DELTA-6 DESATURASE; DIETARY FISH-OIL; SALMO-SALAR L; SKELETAL-MUSCLE; LIPOPROTEIN-LIPASE; ATLANTIC SALMON; RAINBOW-TROUT;
D O I
10.1017/S000711451200311X
中图分类号
R15 [营养卫生、食品卫生]; TS201 [基础科学];
学科分类号
100403 ;
摘要
The effect of ration size on muscle fatty acid (FA) composition and mRNA expression levels of key regulatory enzymes of lipid and lipoprotein metabolism have been addressed in juveniles of gilthead sea bream fed a practical diet over the course of an 11-week trial. The experimental setup included three feeding levels: (i) full ration until visual satiety, (ii) 70% of satiation and (iii) 70% of satiation with the last 2 weeks at the maintenance ration. Feed restriction reduced lipid content of whole body by 30% and that of fillet by 50 %. In this scenario, the FA composition of fillet TAG was not altered by ration size, whereas that of phospholipids was largely modified with a higher retention of arachidonic acid and DHA. The mRNA transcript levels of lysophosphatidylcholine acyltransferases, phosphatidylethanolamine N-methyltransferase and FA desaturase 2 were not regulated by ration size in the present experimental model. In contrast, mRNA levels of stearoyl-CoA desaturases were markedly down-regulated by feed restriction. An opposite trend was found for a muscle-specific lipoprotein lipase, which is exclusive of fish lineage. Several upstream regulatory transcriptions were also assessed, although nutritionally mediated changes in mRNA transcripts were almost reduced to PPAR alpha and beta, which might act in a counter-regulatory way on lipolysis and lipogenic pathways. This gene expression pattern contributes to the construction of a panel of biomarkers to direct marine fish production towards muscle lean phenotypes with increased retentions of long-chain PUFA.
引用
收藏
页码:1175 / 1187
页数:13
相关论文
共 46 条
  • [21] Fatty Acid Composition and Fatty Acid Associated Gene-Expression in Gilthead Sea Bream (Sparus aurata) are Affected by Low-Fish Oil Diets, Dietary Resveratrol, and Holding Temperature
    Torno, Claudia
    Staats, Stefanie
    Michl, Stephanie Celine
    de Pascual-Teresa, Sonia
    Izquierdo, Marisol
    Rimbach, Gerald
    Schulz, Carsten
    MARINE DRUGS, 2018, 16 (10):
  • [22] A transcriptomic approach to study the effect of long-term starvation and diet composition on the expression of mitochondrial oxidative phosphorylation genes in gilthead sea bream (Sparus aurata)
    Jonás I. Silva-Marrero
    Alberto Sáez
    Albert Caballero-Solares
    Ivan Viegas
    María Pilar Almajano
    Felipe Fernández
    Isabel V. Baanante
    Isidoro Metón
    BMC Genomics, 18
  • [23] Liver insulin-like growth factor-I mRNA is not affected by diet composition or ration size but shows diurnal variations in regularly-fed gilthead sea bream (Sparus aurata)
    Metón, I
    Caseras, A
    Cantó, E
    Fernándes, F
    Baanante, IV
    JOURNAL OF NUTRITION, 2000, 130 (04): : 757 - 760
  • [24] THE EFFECTS OF WEANING ON TO A DRY PELLET DIET ON BRAIN LIPID AND FATTY-ACID COMPOSITIONS IN POSTLARVAL GILTHEAD SEA BREAM (SPARUS-AURATA L)
    MOURENTE, G
    TOCHER, DR
    COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY A-PHYSIOLOGY, 1993, 104 (03): : 605 - 611
  • [25] Effects of dietary soybean oil concentration on growth, nutrient utilization and muscle fatty acid composition of gilthead sea bream (Sparus aurata L.)
    Martinez-Llorens, Silvia
    Vidal, Ana Tomas
    Monino, Andres Vicente
    Torres, Marcial Pla
    Cerda, Miguel Jover
    AQUACULTURE RESEARCH, 2007, 38 (01) : 76 - 81
  • [26] Skin Mucus Fatty Acid Composition of Gilthead Sea Bream (Sparus Aurata): A Descriptive Study in Fish Fed Low and High Fish Meal Diets
    Torrecillas, Silvia
    Montero, Daniel
    Dominguez, David
    Robaina, Lidia
    Izquierdo, Marisol
    FISHES, 2019, 4 (01)
  • [27] Effect of a specific composition of short- and medium-chain fatty acid 1-Monoglycerides on growth performances and gut microbiota of gilthead sea bream (Sparus aurata)
    Rimoldi, Simona
    Gliozheni, Emi
    Ascione, Chiara
    Gini, Elisabetta
    Terova, Genciana
    PEERJ, 2018, 6
  • [28] EFFECT OF BROODSTOCK DIETS ON TOTAL LIPIDS AND FATTY-ACID COMPOSITION OF LARVAE OF GILTHEAD SEA BREAM (SPARUS-AURATA L) DURING YOLK-SAC STAGE
    MOURENTE, G
    ODRIOZOLA, JM
    FISH PHYSIOLOGY AND BIOCHEMISTRY, 1990, 8 (02) : 103 - 110
  • [29] Monthly variation of growth, biochemical composition, fatty and amino acids patterns of gilthead sea bream (Sparus aurata) in offshore cage systems under brackish water conditions in the Black Sea
    Ozturk, Dilara Kaya
    Ozturk, Recep
    Baki, Birol
    Karayucel, Ismihan
    Karayucel, Sedat
    AQUACULTURE RESEARCH, 2020, 51 (12) : 4961 - 4983
  • [30] Effects of olive pomace and olive pomace oil on growth performance, fatty acid composition and cardio protective properties of gilthead sea bream (Sparus aurata) and sea bass (Dicentrarchus labrax)
    Nasopoulou, Constantina
    Stamatakis, Giorgos
    Demopoulos, Constantinos A.
    Zabetakis, Ioannis
    FOOD CHEMISTRY, 2011, 129 (03) : 1108 - 1113