Whispering gallery mode aptasensors for detection of blood proteins

被引：38

作者：

Pasquardini, Laura ^{[1
]}

Berneschi, Simone ^{[2
,3
]}

Barucci, Andrea ^{[3
]}

Cosi, Franco ^{[3
]}

Dallapiccola, Ramona ^{[1
]}

Insinna, Massimiliano ^{[5
]}

Lunelli, Lorenzo ^{[1
,4
]}

Conti, Gualtiero Nunzi ^{[3
]}

Pederzolli, Cecilia ^{[1
]}

Salvadori, Simone ^{[5
]}

Soria, Silvia ^{[3
]}

机构：

[1] FBK CMM, I-38123 Trento, Italy

[2] Ctr Studi & Ric E Fermi, I-00184 Rome, Italy

[3] IFAC CNR, Inst Appl Phys, I-50019 Sesto Fiorentino, FI, Italy

[4] IBF CNR, Unit Trento, I-38123 Trento, Italy

[5] Univ Florence, Energy Engn Dept S Stecco, I-50139 Florence, Italy

来源：

JOURNAL OF BIOPHOTONICS | 2013年 / 6卷 / 02期

关键词：

whispering gallery modes; microresonators; aptamers; thrombin; VEGF; human serum; aptasensor; SHIFT; MICROSPHERES;

D O I：

10.1002/jbio.201200013

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Whispering gallery mode resonators (WGMR), as silica microspheres, have been recently proposed as an efficient tool for the realisation of optical biosensors. In this work we present a functionalization procedure based on the DNA-aptamer sequence immobilization on WGMR, able to recognize specifically thrombin or VEGF protein, preserving a high Q factor. The protein binding was optically characterized in terms of specificity in buffer solution or in 10% diluted human serum. Simulation of the protein flow was found in good agreement with experimental data. The aptasensor was also chemically regenerated and tested again, demonstrating the reusability of our system. ((c) 2013 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim)

引用

页码：178 / 187

页数：10

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