Characterizations of coal fly ash nanoparticles and induced in vitro toxicity in cell lines

被引：9
|
作者：
Sambandam, Bharathi [1 ]
Palanisami, Eganathan [2 ]
Abbugounder, Rajasekar [1 ]
Prakhya, Balakrishnamurthy [3 ]
Thiyagarajan, Devasena [1 ]
机构：
[1] Anna Univ, Ctr Nanosci & Technol, Madras 600025, Tamil Nadu, India
[2] MS Swaminathan Res Fdn, Plant Tissue Culture & Bioprospecting Lab, Madras 600113, Tamil Nadu, India
[3] Int Inst Biotechnol & Toxicol IIBAT, Padappai 601301, India
来源：
JOURNAL OF NANOPARTICLE RESEARCH | 2014年 / 16卷 / 02期
关键词：
Coal fly ash; Coal fly ash nanoparticles; MTT assay; Cytotoxicity; Environmental and health effects; LUNG EPITHELIAL-CELLS; DNA-DAMAGE; ULTRAFINE PARTICLES; OXIDATIVE STRESS; COMBUSTION; SIZE; IRON; METALS; CYTOTOXICITY; MOBILIZATION;
D O I：
10.1007/s11051-013-2217-x
中图分类号：
O6 [化学];
学科分类号：
0703 ;
摘要：
The present study illustrates the characterization and cytotoxicity studies of coal fly ash nanoparticles (CFA-NPs). The coal fly ash (CFA) collected from electrostatic precipitator of a coal-fired power plant and the average size of the CFA-NPs was found to be 9-50 nm. Imaging techniques showed predominantly homogenous spherical shaped nanoparticles. The X-ray diffraction analysis and energy dispersive X-ray (EDAX) analysis spectra reveal the elemental constituents of the CFA-NPs contain several toxic heavy metals. Cytotoxicity of CFA-NPs was determined by MTT assay. Cellular metabolism is inhibited in a dose dependent manner by CFA concentrations varying from 13 to 800 mu g mL(-1). After 48 h exposure, the Hep2, A549 and HepG2 cell lines prove more sensitive to CFA-NPs at varying levels which results in IC50 (50 % inhibitory concentration) cytotoxicity end point.
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页数：9
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