Physicochemical properties and antiproliferative activity of recombinant Yersinia pseudotuberculosis L-asparaginase

被引:4
|
作者
Pokrovskii, V. S. [1 ]
Pokrovskaya, M. V. [1 ]
Aleksandrova, S. S. [1 ]
Anrianov, R. M. [2 ]
Zhdanov, D. D. [1 ]
Osmel'yanyuk, N. M. [1 ]
Treshchalina, E. M. [3 ]
Sokolov, N. N. [1 ]
机构
[1] Russian Acad Med Sci, Inst Biomed Chem, Moscow 119832, Russia
[2] Russian Acad Sci, Bach Inst Biochem, Moscow 117071, Russia
[3] Russian Acad Med Sci, Canc Res Ctr, Moscow 115478, Russia
关键词
CELL LYMPHOMA; GLUTAMINASE;
D O I
10.1134/S000368381301016X
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
The physicochemical, catalytic, and antiproliferative activity of a recombinant L-asparaginase from Yersinia pseudotuberculosis (YpA) have been studied. The following results were obtained: the K (M) value for L-asparagine is 17 +/- 0.9 mu M, the optimal temperature is 60A degrees C, pH is 8.0, pI is 5.4 +/- 0.3, the L-glutaminase activity is no more than 5-6% of the L-asparaginase activity, and the antiproliferative activity on the Fisher L5178y lymphadenosis cell line comprised T/C = 136% (p < 0.001) at a 15% recovery rate. The described characteristic allows one to regard YpA as an antitumor enzyme with biological features similar to the L-asparaginase of E. coli.
引用
收藏
页码:18 / 22
页数:5
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