Physicochemical properties and antiproliferative activity of recombinant Yersinia pseudotuberculosis L-asparaginase

被引：4

作者：

Pokrovskii, V. S. ^{[1
]}

Pokrovskaya, M. V. ^{[1
]}

Aleksandrova, S. S. ^{[1
]}

Anrianov, R. M. ^{[2
]}

Zhdanov, D. D. ^{[1
]}

Osmel'yanyuk, N. M. ^{[1
]}

Treshchalina, E. M. ^{[3
]}

Sokolov, N. N. ^{[1
]}

机构：

[1] Russian Acad Med Sci, Inst Biomed Chem, Moscow 119832, Russia

[2] Russian Acad Sci, Bach Inst Biochem, Moscow 117071, Russia

[3] Russian Acad Med Sci, Canc Res Ctr, Moscow 115478, Russia

来源：

APPLIED BIOCHEMISTRY AND MICROBIOLOGY | 2013年 / 49卷 / 01期

关键词：

CELL LYMPHOMA; GLUTAMINASE;

D O I：

10.1134/S000368381301016X

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

The physicochemical, catalytic, and antiproliferative activity of a recombinant L-asparaginase from Yersinia pseudotuberculosis (YpA) have been studied. The following results were obtained: the K (M) value for L-asparagine is 17 +/- 0.9 mu M, the optimal temperature is 60A degrees C, pH is 8.0, pI is 5.4 +/- 0.3, the L-glutaminase activity is no more than 5-6% of the L-asparaginase activity, and the antiproliferative activity on the Fisher L5178y lymphadenosis cell line comprised T/C = 136% (p < 0.001) at a 15% recovery rate. The described characteristic allows one to regard YpA as an antitumor enzyme with biological features similar to the L-asparaginase of E. coli.

引用

页码：18 / 22

页数：5

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