Study on viability and chondrogenic differentiation of cryopreserved adipose tissue-derived mesenchymal stromal cells for future use in regenerative medicine

被引：21
|
作者：
Gonzalez-Fernandez, M. L. [1 ]
Perez-Castrillo, S. [1 ]
Ordas-Fernandez, P. [1 ]
Lopez-Gonzalez, M. E. [1 ]
Colaco, B. [2 ]
Villar-Suarez, V. [3 ]
机构：
[1] Univ Leon, Fac Vet Med, Dept Anat, Leon 24008, Spain
[2] Univ Tros os Montes & Alto Douro, Zootecnia Dept, CECAV, P-5001801 Vila Real, Portugal
[3] Univ Leon, Fac Vet Med, Biomed Inst, Leon 24008, Spain
来源：
CRYOBIOLOGY | 2015年 / 71卷 / 02期
关键词：
Adipose derived mesenchymal stromal cells; Cryopreservation; Chondrogenic differentiation; UMBILICAL-CORD BLOOD; STEM-CELLS; BONE-MARROW; PROGENITOR CELLS; IN-VITRO; TREHALOSE; MULTIPOTENCY; CAPACITY; INFUSION; DMSO;
D O I：
10.1016/j.cryobiol.2015.07.007
中图分类号：
Q [生物科学];
学科分类号：
07 ; 0710 ; 09 ;
摘要：
Adipose-derived mesenchymal stromal cells are promising as a regenerative therapy tool for defective tissues in mesenchymal lineage, including fat, bone, cartilage, and blood vessels. In potential future clinical applications, adipose-derived stem cell cryopreservation is an essential fundamental technology. The aim of this study is to define an adequate protocol for the cryopreservation of adipose-derived mesenchymal stromal cells, by comparing various protocols so as to determine the effects of cryopreservation on viability and chondrogenic differentiation potential of adipose-derived stem cells upon freeze-thawing of AT-MSCs colonies cryopreserved with standard and modified protocols, using flow cytometry and confocal microscopy. The study concludes that adipose-derived mesenchymal stromal cells could be long-term cryopreserved without any loss of their proliferative or differentiation potential. (C) 2015 Elsevier Inc. All rights reserved.
引用
收藏
页码：256 / 263
页数：8
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