BLIND DECONVOLUTION OF 3D DATA IN WIDE FIELD FLUORESCENCE MICROSCOPY

被引：0

作者：

Soulez, Ferreol ^{[1
]}

Denis, Loic ^{[2
,3
]}

Tourneur, Yves ^{[1
]}

Thiebaut, Eric ^{[2
]}

机构：

[1] Univ Lyon 1, Ctr Commun Quantimetrie, 8 Ave Rockefeller, F-69373 Lyon 08, France

[2] Univ Lyon 1, ENS Lyon, Ctr Rech Astrophys Lyon, CNRS UMR 5574, F-69561 St Genis Laval, France

[3] Univ Jean Monnet, CNRS, UMR 5516, Lab Hubert Curien, F-42000 St Etienne, France

来源：

2012 9TH IEEE INTERNATIONAL SYMPOSIUM ON BIOMEDICAL IMAGING (ISBI) | 2012年

关键词：

blind deconvolution; wide field fluorescence microscopy; image restoration; inverses problems; IMAGE; ALGORITHMS;

D O I：

暂无

中图分类号：

TM [电工技术]; TN [电子技术、通信技术];

学科分类号：

0808 ; 0809 ;

摘要：

In this paper we propose a blind deconvolution algorithm for wide field fluorescence microscopy. The 3D PSF is modeled after a parametrized pupil function. The PSF parameters are estimated jointly with the object in a maximum a posteriori framework. We illustrate the performances of our algorithm on experimental data and show significant resolution improvement notably along the depth. Quantitative measurements on images of calibration beads demonstrate the benefits of blind deconvolution both in terms of contrast and resolution compared to non-blind deconvolution using a theoretical PSF.

引用

页码：1735 / 1738

页数：4

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