An alternative method to prepare samples of the pathogenic yeast Cryptococcus neoformans for scanning electron microscopy analysis

被引：6
|
作者：
Faganello, J
Arruda, W
Schrank, A
Vainstein, MH
机构：
[1] Univ Fed Rio Grande do Sul, Ctr Biotecnol, BR-91501970 Porto Alegre, RS, Brazil
[2] Univ Fed Rio Grande do Sul, Inst Biociencias, Dept Biol Mol & Biotecnol, BR-91501970 Porto Alegre, RS, Brazil
[3] Univ Fed Rio Grande do Sul, Inst Ciencias Basicas Saude, Dept Microbiol, BR-91501970 Porto Alegre, RS, Brazil
来源：
JOURNAL OF MICROBIOLOGICAL METHODS | 2006年 / 64卷 / 03期
关键词：
SEM; electron microscopy; Cryptococcus neoformans; morphology; fixation; dehydration;
D O I：
10.1016/j.mimet.2005.07.003
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
In this work, an alternative to conventional preparation procedures for scanning electron microscopy (SEM) analysis of Cryptococcus neoformans was performed. The cells were fixed directly in the agar culture. This method is simpler than others already reported and the morphology of the cells was well preserved. (C) 2005 Elsevier B.V All rights reserved.
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页码：416 / 419
页数：4
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