Preparation and Electrochemical Behavior of L-Glutamate Electrochemical Biosensor

被引：253
|
作者：
Deng, Yan [1 ,2 ]
Wang, Wei [1 ]
Zhang, Liming [1 ,2 ]
Lu, Zhuoxuan [1 ,2 ]
Li, Song [1 ]
Xu, Lijian [1 ]
机构：
[1] Hunan Univ Technol, Hunan Key Lab Green Packaging & Applicat Biol Nan, Zhuzhou 412007, Peoples R China
[2] Southeast Univ, State Key Lab Bioelect, Nanjing 210096, Jiangsu, Peoples R China
来源：
JOURNAL OF BIOMEDICAL NANOTECHNOLOGY | 2013年 / 9卷 / 02期
关键词：
L-Glutamate; Biosensor; Electrochemical; Nano-Porous Pseudo Carbon Paste Electrode; CARBON NANOTUBES; MAGNETIC NANOPARTICLES; MASS-SPECTROMETRY; ACID; OXIDASE; SYSTEM; CANCER; ZNO;
D O I：
10.1166/jbn.2013.1487
中图分类号：
TB3 [工程材料学];
学科分类号：
0805 ; 080502 ;
摘要：
Using the nano-porous pseudo carbon paste electrode (Nano-PPCPE) as the working electrode, mixing L-glutamate oxidase (L-GLOD), catalase (Cat) and bovine serum albumin (BSA) with phosphate buffer (PB, pH = 7.4), followed by cross-linking with glutaraldehyde, a novel L-glutamate electrochemical biosensor was successfully formed. It was demonstrated that the modified nano-PPCPE exhibits a high selectivity and sensitivity in comparison with the modified CPE. The L-glutamate biosensor showed a linear range from 5 x 10(-7) M to 1 x 10(-5) M with the detection limit of 2.5 x 10(-7) M.
引用
收藏
页码：318 / 321
页数：4
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