Detection of marine pathogenic bacterial Vibrio species by multiplex polymerase chain reaction (PCR)

被引：0

作者：

Kim, Mal Nam ^{[1
]}

Bang, Hyo Joo ^{[1
]}

机构：

[1] Sangmyung Univ, Dept Biol, Seoul 110743, South Korea

来源：

JOURNAL OF ENVIRONMENTAL BIOLOGY | 2008年 / 29卷 / 04期

关键词：

Vibrio sp; multiplex PCR; species-specific primer;

D O I：

暂无

中图分类号：

X [环境科学、安全科学];

学科分类号：

08 ; 0830 ;

摘要：

Polymerase chain reactions (PCR) were performed to accomplish quick and accurate detection of Vibrio species. The primers prepared with 16S-23S rDNA intergenic spacer (IGS) region exhibited an excellent species-specificity for Vibrio sp and detected Vibrio sp more successfully than the con ventional culture method. Multiplex PCR was also fruitful not only for the identification of the 5 standard Vibrio sp simultaneously but also for the detection of Vibrio sp in the samples collected from the natural environment.

引用

页码：543 / 546

页数：4

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