Detection of marine pathogenic bacterial Vibrio species by multiplex polymerase chain reaction (PCR)

Polymerase chain reactions (PCR) were performed to accomplish quick and accurate detection of Vibrio species. The primers prepared with 16S-23S rDNA intergenic spacer (IGS) region exhibited an excellent species-specificity for Vibrio sp and detected Vibrio sp more successfully than the con ventional culture method. Multiplex PCR was also fruitful not only for the identification of the 5 standard Vibrio sp simultaneously but also for the detection of Vibrio sp in the samples collected from the natural environment.