Simultaneous Determination of Artemether and Its Major Metabolites Dihydroartimisinin in Rat Plasma Using Ultra Performance Liquid Chromatography-Tandem Mass Spectrometry

被引：5

作者：

Li Chun ^{[1
,2
]}

Qiu Yu-Qin ^{[1
]}

Yang Guo-Zhong ^{[1
,2
]}

Meng Fan-Da ^{[1
,2
]}

Zhang Suo-Hui ^{[1
]}

Gao Yun-Hua ^{[1
]}

机构：

[1] Chinese Acad Sci, Inst Phys & Chem Technol, Key Lab Photochem Convers & Funct Mat, Beijing 100190, Peoples R China

[2] Univ Chinese Acad Sci, Beijing 100049, Peoples R China

来源：

CHINESE JOURNAL OF ANALYTICAL CHEMISTRY | 2015年 / 43卷 / 04期

关键词：

Artemether; Dihydroartemisinin; Orbitrap; Rat plasma; Pharmacokinetics; DIHYDROARTEMISININ; VALIDATION;

D O I：

10.11895/j.issn.0253-3820.141095

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

In order to study the pharmacokinetics of artemether in rats, artemether and its major metabolites dihydroartimisinin in rat plasma were simultaneously determined by an ultra performance liquid chromatography coupled with quadrupole-obitrap mass spectrometric method with high sensitivity. Artemisinin was used as internal standard. Identification and quantification of artemether and dihydroartimisinin were performed with electrospray ionization under a positive Targeted-MS2 monitoring mode. The monitored transitions were m/z 316.2115/163.1117 (artemether), m/z 302.1958/163.1117 (dihydroartimisinin) and m/z 300.1803/209.1536 (artemisinin). A good linearity was obtained in the range of 2-200 mu g/L (R-2 > 0.9990) with a limit of detection of 0.8 mu g/L and a limit of quantification of 2.0 mu g/L for both artemether and dihydroartimisinin. The recoveries of artemether and dihydroartimisinin were 93.7% - 103.7% and 97.4% - 104.7%, respectively. The RSD was less than 9%. The method is rapid, sensitive, reproducible, and can be applied in pharmacokinetic studies of artemether in rats.

引用

页码：560 / 565

页数：6