Comparison of bone tissue properties in mouse models with collagenous and non-collagenous genetic mutations using FTIRI

被引:33
|
作者
Coleman, Rhima M. [1 ]
Aguilera, Laura [2 ]
Quinones, Layla [2 ]
Lukashoya, Lyudamila [1 ]
Poirier, Christophe [3 ]
Boskey, Adele [1 ]
机构
[1] Cornell Univ, Hosp Special Surg, Weill Med Coll, New York, NY 10021 USA
[2] CUNY, LaGuardia Community Coll, New York, NY 10021 USA
[3] Indiana Univ, Dept Med, Indianapolis, IN USA
关键词
Osteogenesis imperfecta; Mouse models; Fourier transform infrared spectroscopy; Microcomputed tomography; FRAGILITAS-OSSIUM FRO/FRO; OSTEOGENESIS IMPERFECTA; NEUTRAL SPHINGOMYELINASE; MINERAL CRYSTALLINITY; RECESSIVE MUTATION; CROSS-LINKS; PROTEOGLYCANS; DEFICIENCY; FIBRILS; SMPD3;
D O I
10.1016/j.bone.2012.08.110
中图分类号
R5 [内科学];
学科分类号
1002 ; 100201 ;
摘要
Understanding how the material properties of bone tissue from the various forms of oSteogenesis imperfecta (01) differ will allow us to tailor treatment regimens for affected patients. To this end, we characterized the bone structure and material properties of two mouse models of Cl, the osteogenesis imperfecta mouse (oim/ oim) and fragilitas ossium (fro/fro), in which bone fragility is due to a genetic defect in collagen type land a defect in osteoblast matrix mineralization, respectively. Bones from 3 to 6 month old animals were examined using Fourier transform infrared spectroscopic imaging (FTIRI), microcomputed tomography (micro-CT), histology, and biochemical analysis. The attributes of oim/oim bone tissue were relatively constant over time when compared to wild type animals. The mineral density in oim/oim cortices and trabecular bone was higher than wild type while the bones had thinner cortices and fewer trabeculae that were thinner and more widely spaced. The fro/fro animals exhibited osteopenic attributes at 3 months. However, by 6 months, their spectroscopic and geometric properties were similar to wild type animals. Despite the lack of a specific collagen defect in fro/fro mice, both fro/fro and oim/oim genotypes exhibited abnormal collagen crosslinking as determined by FTIRI at both time points. These results demonstrate that abnormal extracellular matrix assembly plays a role in the bone fragility in both of these models. (C) 2012 Elsevier Inc. All rights reserved.
引用
收藏
页码:920 / 928
页数:9
相关论文
共 50 条
  • [1] STUDIES ON NON-COLLAGENOUS PROTEINS OF BONE
    ASHTON, BA
    HERRING, GM
    OWEN, ME
    TRIFFITT, IT
    ISRAEL JOURNAL OF MEDICAL SCIENCES, 1971, 7 (03): : 409 - &
  • [2] NON-COLLAGENOUS PROTEINS OF RAT BONE
    LINDE, A
    JONTELL, M
    LUNDGREN, T
    NILSON, B
    JOURNAL OF DENTAL RESEARCH, 1983, 62 (04) : 487 - 487
  • [3] Comparison of collagenous and non-collagenous membranes implanted subcutaneously in monkeys.
    Siar, CH
    Ng, KH
    Romanos, GE
    Toh, CG
    JOURNAL OF DENTAL RESEARCH, 2003, 82 : 664 - 664
  • [4] Collagenous and non-collagenous biochemical markers of bone metastases from prostate cancer
    Zafeirakis, A.
    HIPPOKRATIA, 2010, 14 (03) : 164 - 169
  • [5] NON-COLLAGENOUS PROTEINS OF BONE - POTENTIAL FUNCTIONS AND USES
    TRIFFITT, JT
    BONE, 1988, 9 (04) : 259 - 260
  • [6] MONOCLONAL-ANTIBODIES TO NON-COLLAGENOUS BONE PROTEINS
    STENNER, DD
    ROMBERG, RW
    TRACY, RP
    KATZMANN, JA
    RIGGS, BL
    MANN, KG
    CALCIFIED TISSUE INTERNATIONAL, 1983, 35 (4-5) : 665 - 665
  • [7] NON-COLLAGENOUS PROTEINS OF RAT COMPACT-BONE
    LINDE, A
    JONTELL, M
    LUNDGREN, T
    NILSON, B
    SVANBERG, U
    JOURNAL OF BIOLOGICAL CHEMISTRY, 1983, 258 (03) : 1698 - 1705
  • [8] ANTIGENICITY OF NON-COLLAGENOUS PROTEINS OF HUMAN-BONE
    QUELCH, KJ
    MELICK, RA
    CONNECTIVE TISSUE RESEARCH, 1981, 8 (02) : 142 - 142
  • [9] NON-COLLAGENOUS PROTEINS ASSOCIATED WITH BONE MORPHOGENETIC ACTIVITY
    MIKULSKI, AJ
    BUTLER, W
    URIST, MR
    JOURNAL OF DENTAL RESEARCH, 1976, 55 : B75 - B75
  • [10] Osteogenesis imperfecta due to mutations in non-collagenous genes: lessons in the biology of bone formation
    Marini, Joan C.
    Reich, Adi
    Smith, Simone M.
    CURRENT OPINION IN PEDIATRICS, 2014, 26 (04) : 500 - 507