Ultraviolet-Visible (UV-Vis) and Fluorescence Spectroscopic Investigation of the Interactions of Ionic Liquids and Catalase

被引：11
|
作者：
Dong, Xing [1 ]
Fan, Yunchang [1 ]
Yang, Peng [1 ]
Kong, Jichuan [1 ]
Li, Dandan [1 ]
Miao, Juan [1 ]
Hua, Shaofeng [1 ]
Hu, Chaobing [1 ]
机构：
[1] Henan Polytech Univ, Coll Phys & Chem, Jiaozuo 454003, Peoples R China
来源：
APPLIED SPECTROSCOPY | 2016年 / 70卷 / 11期
基金：
中国国家自然科学基金;
关键词：
Ionic liquids; ILs; fluorescence spectroscopy; catalase; hydrogen bonding; hydrophobicity; HUMAN SERUM-ALBUMIN; CYTOCHROME-C; 1-BUTYL-3-METHYLIMIDAZOLIUM CHLORIDE; TOXICITY ASSESSMENT; HOFMEISTER SERIES; AQUEOUS-SOLUTIONS; VIBRIO-FISCHERI; STABILITY; PROTEIN; ENZYME;
D O I：
10.1177/0003702816653124
中图分类号：
TH7 [仪器、仪表];
学科分类号：
0804 ; 080401 ; 081102 ;
摘要：
The inhibitory effects of nine ionic liquids (ILs) on the catalase activity were investigated using fluorescence, absorption ultraviolet-visible spectroscopy. The interactions of ILs and catalase on the molecular level were studied. The experimental results indicated that ILs could inhibit the catalase activity and their inhibitory abilities depended on their chemical structures. Fluorescence experiments showed that hydrogen bonding played an important role in the interaction process. The inhibitory abilities of ILs on catalase activity could be simply described by their hydrophobicity and hydrogen bonding abilities. Unexpected less inhibitory effects of trifluoromethanesulfonate (TfO-) might be ascribed to its larger size, which makes it difficult to go through the substrate channel of catalase to the active site.
引用
收藏
页码：1851 / 1860
页数：10
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