Screening for anabolic steroids in doping analysis by liquid chromatography/electrospray ion trap mass spectrometry

被引：53

作者：

Deventer, K. ^{[1
]}

Van Eenoo, P. ^{[1
]}

Delbeke, F. T. ^{[1
]}

机构：

[1] Univ Ghent, Dept Clin Chem Microbiol & Immunol, DoCoLab, B-9052 Zwijnaarde, Belgium

来源：

BIOMEDICAL CHROMATOGRAPHY | 2006年 / 20卷 / 05期

关键词：

anabolic steroids; stanozolol; 3 '-hydroxystanozolol; gestrinone; 17; alpha-trenbolone; doping analysis;

D O I：

10.1002/bmc.579

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A fast and selective LC/MS/MS method for the screening of four anabolic steroids in human urine has been developed and validated. Liquid-liquid extraction with diethyl ether was applied after enzymatic hydrolysis. Analyses were performed on an ion trap mass spectrometer equipped with electrospray ionisation. MS/MS was applied for all compounds. The analytical run time was 11 min. ne LOD for all compounds varied between 1 and 10 ng/mL. Left-over A samples, which were declared positive by GC/MS for the presence of 3'-hydroxystanozolol, were assessed using the described method. Copyright (c) 2005 John Wiley & Sons, Ltd.

引用

页码：429 / 433

页数：5

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