Screening for anabolic steroids in doping analysis by liquid chromatography/electrospray ion trap mass spectrometry

被引：53
|
作者：
Deventer, K. [1 ]
Van Eenoo, P. [1 ]
Delbeke, F. T. [1 ]
机构：
[1] Univ Ghent, Dept Clin Chem Microbiol & Immunol, DoCoLab, B-9052 Zwijnaarde, Belgium
来源：
BIOMEDICAL CHROMATOGRAPHY | 2006年 / 20卷 / 05期
关键词：
anabolic steroids; stanozolol; 3 '-hydroxystanozolol; gestrinone; 17; alpha-trenbolone; doping analysis;
D O I：
10.1002/bmc.579
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
A fast and selective LC/MS/MS method for the screening of four anabolic steroids in human urine has been developed and validated. Liquid-liquid extraction with diethyl ether was applied after enzymatic hydrolysis. Analyses were performed on an ion trap mass spectrometer equipped with electrospray ionisation. MS/MS was applied for all compounds. The analytical run time was 11 min. ne LOD for all compounds varied between 1 and 10 ng/mL. Left-over A samples, which were declared positive by GC/MS for the presence of 3'-hydroxystanozolol, were assessed using the described method. Copyright (c) 2005 John Wiley & Sons, Ltd.
引用
收藏
页码：429 / 433
页数：5
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