Genetic identification of cinnamon (Cinnamomum spp.) based on the trnL-trnF chloroplast DNA

被引：35
|
作者：
Kojoma, M [1 ]
Kurihara, K
Yamada, K
Sekita, S
Satake, M
Iida, O
机构：
[1] Natl Inst Hlth Sci, Izu Expt Stn Med Plants, Shizuoka, Japan
[2] Natl Inst Hlth Sci, Div Pharmacognosy & Phytochem, Tokyo 158, Japan
[3] RIKEN, Inst Phys & Chem Res, Wako, Saitama 35101, Japan
来源：
PLANTA MEDICA | 2002年 / 68卷 / 01期
关键词：
D O I：
10.1055/s-2002-20051
中图分类号：
Q94 [植物学];
学科分类号：
071001 ;
摘要：
Genetic identification among cinnamon species was studied by analyzing nucleotide sequences of chloroplast DNA from four species (Cinnamomum cassia, C. zeylanicum, C. burmannii and C. sieboldii). The two regions studied were the intergenic spacer region between the trnL 3'exon and trnF exon (trnL -trnF IGS) and the trnL intron region, We found nucleotide variation at one site in the trnL-trnF IGS, and at three sites in the trnL intron. With the sequence data from analysis of these regions, the four Cinnamomum species used in this study were correctly identified. Furthermore, single-strand conformation polymorphism (SSCP) analysis of PCR products from the trnL-trnF IGS and the trnL intron resulted in different SSCP band patterns among C. cassia, C. zeylanicum and C. burmannii.
引用
收藏
页码：94 / 96
页数：3
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