Functional analysis of the conserved histidine residue of Bamboo mosaic virus capping enzyme in the activity for the formation of the covalent enzyme-m7GMP intermediate

被引：8

作者：

Lin, Hua-Yang ^{[1
]}

Yu, Chiao-Yuan ^{[1
]}

Hsu, Yau-Heiu ^{[1
]}

Meng, Menghsiao ^{[1
]}

机构：

[1] Natl Chung Hsing Univ, Grad Inst Biotechnol, Taichung 40227, Taiwan

来源：

FEBS LETTERS | 2012年 / 586卷 / 16期

关键词：

Bamboo mosaic virus; Alphavirus-like superfamily; Hydroxylamine; Capping enzyme; Guanylyltransferase; VESICULAR STOMATITIS-VIRUS; SACCHAROMYCES-CEREVISIAE; DEPENDENT GUANYLYLTRANSFERASE; S-ADENOSYLMETHIONINE; NUCLEOTIDYL TRANSFER; RNA-POLYMERASE; PROTEIN; DOMAIN; SITE; METHYLTRANSFERASE;

D O I：

10.1016/j.febslet.2012.05.024

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The alphavirus-like mRNA capping enzyme of Bamboo mosaic virus (BaMV) exhibits an AdoMet-dependent guanylyltransferase activity by which the methyl group of AdoMet is transferred to GTP, leading to the formation of m(7)GTP, and the m(7)GMP moiety is next transferred to the 5' end of ppRNA via a covalent enzyme-m(7)GMP intermediate. The function of the conserved H68 of the BaMV capping enzyme in the intermediate formation was analyzed by mutagenesis in this study. The nature of the bond linking the enzyme and m(7)GMP was changed in the H68C mutant protein, strongly suggesting that H68 covalently binds to m(7)GMP in the intermediate. (C) 2012 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.

引用

页码：2326 / 2331

页数：6

共 3 条

[1] Critical residues for GTP methylation and formation of the covalent m7GMP-enzyme intermediate in the capping enzyme domain of Bamboo mosaic virus
Huang, YL
Han, YT
Chang, YT
Hsu, YH
Meng, MS
JOURNAL OF VIROLOGY, 2004, 78 (03) : 1271 - 1280
[2] Virus-specific capping of tobacco mosaic virus RNA:: methylation of GTP prior to formation of covalent complex p126-m7GMP
Merits, A
Kettunen, R
Mäkinen, K
Lampio, A
Auvinen, P
Kääriäinen, L
Ahola, T
FEBS LETTERS, 1999, 455 (1-2) : 45 - 48
[3] COVALENT CATALYSIS IN NUCLEOTIDYL TRANSFER - A KTDG MOTIF ESSENTIAL FOR ENZYME-GMP COMPLEX-FORMATION BY MESSENGER-RNA CAPPING ENZYME IS CONSERVED AT THE ACTIVE-SITES OF RNA AND DNA LIGASES
CONG, PJ
SHUMAN, S
JOURNAL OF BIOLOGICAL CHEMISTRY, 1993, 268 (10) : 7256 - 7260

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