Nanotechnology-based cell sheet engineering for regenerative medicine

被引：0

作者：

Yamato, M ^{[1
]}

机构：

[1] Tokyo Womens Med Univ, Inst Adv Biomed Engn & Sci, Shinjuku Ku, Tokyo 1628666, Japan

来源：

Proceedings of the 2005 International Symposium on Micro-NanoMechatronics and Human Science: FROM MICRO & NANO SCALE SYSTEMS TO ROBOTICS & MECHATRONICS SYSTEMS | 2005年

关键词：

RESPONSIVE CULTURE SURFACES; REDUCING TEMPERATURE; TISSUE; RECONSTRUCTION; GRAFTS; SHAPE;

D O I：

暂无

中图分类号：

TH [机械、仪表工业];

学科分类号：

0802 ;

摘要：

In order to avoid several complications resulted from biodegradable scaffolds or single cell injection, we have developed "cell sheet engineering" by utilizing nanotechnology-based temperature-responsive culture dishes. Since temperature-responsive polymers are covalently grafted on the dishes, various types of cells adhere and proliferate on them at 37 degrees C, but are spontaneously detached only by reducing temperature below 32 degrees C without any need for proteolytic enzyme. All the confluent cells are noninvasively harvested as a single contiguous cell sheets with intact cell-cell junctions and deposited extracellular matrix. We have utilized these harvested cell sheets for various tissue reconstruction including ocular surfaces, periodontal ligament, cardiac patches as well as bladder.

引用

页码：251 / 254

页数：4

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