Isolation and characterization of microsatellites in Chinese sturgeon, Acipenser sinensis

被引：57
|
作者：
Zhu, B
Liao, X
Shao, Z
Rosenthal, H
Chang, J
机构：
[1] Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China
[2] World Surg Conservat Soc, D-21629 New Wulmstorf, Germany
来源：
MOLECULAR ECOLOGY NOTES | 2005年 / 5卷 / 04期
关键词：
Acipenser sinensis; microsatellites; Yangtze river;
D O I：
10.1111/j.1471-8286.2005.01100.x
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
From (GATA)(n) and (AAAG)(n) enriched genomic libraries for the Chinese sturgeon (Acipenser sinensis), 50 primer pairs were developed using the fast isolation by AFLP of sequences containing repeats (FIASCO) protocol. Forty-six primer pairs exhibited highly polymorphic with two to 11 alleles per locus, while the rest four displayed monomorphic. These markers yielded 246 alleles in a survey of eight specimens of wild A. sinensis. Average observed heterozygosity ranged from 0.13 to 1.00. These loci should provide sufficient levels of genetic diversity to allow parentage analysis for artificial stocking management and delineation of fine-scale population structure.
引用
收藏
页码：888 / 892
页数：5
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