Cytotoxicity and Glycan-Binding Properties of an 18 kDa Lectin Isolated from the Marine Sponge Halichondria okadai

被引：28
|
作者：
Matsumoto, Ryo [1 ]
Fujii, Yuki [1 ,2 ,6 ]
Kawsar, Sarkar M. A. [1 ,3 ]
Kanaly, Robert A. [1 ]
Yasumitsu, Hidetaro [1 ]
Koide, Yasuhiro [1 ]
Hasan, Imtiaj [1 ]
Iwahara, Chihiro [1 ]
Ogawa, Yukiko [2 ,6 ]
Im, Chang Hun [4 ]
Sugawara, Shigeki [4 ]
Hosono, Masahiro [4 ]
Nitta, Kazuo [4 ]
Hamako, Jiharu [5 ]
Matsui, Taei [5 ]
Ozeki, Yasuhiro [1 ]
机构：
[1] Yokohama City Univ, Grad Sch NanoBio Sci, Dept Genome Syst Sci, Lab Glycobiol & Marine Biochem,Kanazawa Ku, Yokohama, Kanagawa 2360027, Japan
[2] Nagasaki Int Univ, Fac Pharmaceut Sci, Dept Pharm, Div Funct Morphol, Nagasaki 8593298, Japan
[3] Univ Chittagong, Fac Sci, Dept Chem, Lab Carbohydrate & Prot Chem, Chittagong 4221, Bangladesh
[4] Tohoku Pharmaceut Univ, Inst Mol Biomembrane & Glycobiol, Div Cell Recognit Study, Aoba Ku, Sendai, Miyagi 9818558, Japan
[5] Fujita Hlth Univ, Dept Biol, Toyoake, Aichi 4701192, Japan
[6] Nagasaki Int Univ, Div Microbiol, Dept Pharm, Fac Pharmaceut Sci, Nagasaki 8593298, Japan
来源：
TOXINS | 2012年 / 4卷 / 05期
基金：
日本学术振兴会;
关键词：
cytotoxicity; frontal affinity chromatography technology; glycoprotein Japanese black sponge (Halichondria okadai); lectin; PROTEIN; CELLS; OLIGOSACCHARIDE; SILICATEIN; ACID;
D O I：
10.3390/toxins4050323
中图分类号：
TS2 [食品工业];
学科分类号：
0832 ;
摘要：
A divalent cation-independent lectin-HOL-18, with cytotoxic activity against leukemia cells, was purified from a demosponge, Halichondria okadai. HOL-18 is a 72 kDa tetrameric lectin that consists of four non-covalently bonded 18 kDa subunits. Hemagglutination activity of the lectin was strongly inhibited by chitotriose (GlcNAc beta 1-4GlcNAc beta 1-4GlcNAc), fetuin and mucins from porcine stomach and bovine submaxillary gland. Lectin activity was stable at pH 4-12 and temperatures lower than 60 degrees C. Frontal affinity chromatography with 16 types of pyridylaminated oligosaccharides indicated that the lectin had an affinity for N-linked complex-type and sphingolipid-type oligosaccharides with N-acetylated hexosamines and neuramic acid at the non-reducing termini. The lectin killed Jurkat leukemia T cells and K562 erythroleukemia cells in a dose- and carbohydrate-dependent manner.
引用
收藏
页码：323 / 338
页数：16
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