Effects of cerous nitrate on growth and tanshinone production in salvia miltiorrhiza hairy roots

被引：17
|
作者：
Han Mingyu [1 ,2 ]
Guo Wanli [1 ,2 ]
Liang Zongsuo [1 ,2 ]
Yang Dongfeng [1 ,2 ]
Yan Xijun [3 ]
Zhu Yonghong [3 ]
Liu Yan [3 ]
机构：
[1] Zhejiang Sci Tech Univ, Coll Life Sci, Hangzhou 310018, Zhejiang, Peoples R China
[2] Key Lab Plant Secondary Metab & Regulat Zhejiang, Hangzhou 310018, Zhejiang, Peoples R China
[3] Tasly Holding Grp Co Ltd, Tasly R&D Inst, Tianjin 300410, Peoples R China
来源：
JOURNAL OF RARE EARTHS | 2015年 / 33卷 / 11期
关键词：
tanshinones; Salvia miltiorrhiza; Ce3+; secondary metabolism; rare earth element; ISOPRENOID BIOSYNTHESIS; LIMITING ENZYME; CELL-CULTURES; EXPRESSION; SYNTHASE; CLONING; YEAST; REDUCTOISOMERASE; ACCUMULATION; ENHANCEMENT;
D O I：
10.1016/S1002-0721(14)60549-4
中图分类号：
O69 [应用化学];
学科分类号：
081704 ;
摘要：
Salvia miltiorrhiza, a popular traditional Chinese medicine, is widely used for treatments in cardiotonic disease. Tanshinones are a group of bioactive ingredients in S. miltiorrhiza. In this study, Ce3+ was used as an elicitor to enhance tanshinones production in S. miltiorrhiza hairy roots. The results showed that contents of dihydrotanshinone I (DTI) and cryptotanshinone (CT) were significantly enhanced by 50 mu mol/L Ce3+, and reached to 0.875 mg/g and 0.271 mg/g, respectively. However, tanshinone II A (TIIA) and tanshinone I (TI) contents were significantly decreased to 59% and 62% of the control. Simultaneously, expressions of genes (HMGR, DXR, DXS1, DXS2 and GGPPS) involved in tanshinone biosynthesis were upregulated by Ce3+. Responses of DXS1, DXS2 and GGPPS to Ce3+ treatments were later than HMGR and DXR. We speculated that branch pathways of DTI and CT biosynthesis were probably different from TIIA and TI. This work will help us understand biosynthetic mechanism of tanshinones in plants.
引用
收藏
页码：1228 / 1235
页数：8
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