Kinetic method for acetylsalicylic acid determination based on its inhibitory effect upon the catalytic decomposition of H2O2

被引：4
|
作者：
Muresanu, C [1 ]
Copolovici, L [1 ]
机构：
[1] Univ Babes Bolyai, Fac Chem & Chem Engn, R-3400 Cluj Napoca, Romania
来源：
ANALYTICAL AND BIOANALYTICAL CHEMISTRY | 2004年 / 378卷 / 07期
关键词：
acetylsalicylic acid; catalase; hydrogen peroxide; inhibition; kinetic methods;
D O I：
10.1007/s00216-003-2470-4
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
The catalytic reaction of catalase was investigated, by means of a Clark oxygen sensor, in the presence of various concentrations of acetylsalicylic acid. Michaelis-Menten kinetic parameters were determined from Lineweaver-Burk plots, obtained in the absence and in the presence of the inhibitor. The inhibition pattern, suggested by the Lineweave-Burk plots, corresponds to a fully mixed inhibition mechanism. A kinetic method, based on the indicator reaction: H2O2 --> catalase, acetylsalicylic acid H2O + 0.5O(2), was developed for the quantitative determination of acetylsalicylic acid. Calibration graphs of the reciprocal value of first-order rate constant versus acetylsalicylic concentration covered the concentration range (2.99-19.98)x10(-4) mol/L, while the detection limit was 4.12x10(-4) mol/L acetylsalicylic acid with a standard deviation of 2.1x10(-5) mol/L.
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页码：1868 / 1872
页数：5
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