Chemical probes for profiling fatty acid-associated proteins in living cells

被引：12

作者：

Raghavan, Anuradha ^{[1
]}

Charron, Guillaume ^{[1
]}

Flexner, James ^{[1
]}

Hang, Howard C. ^{[1
]}

机构：

[1] Rockefeller Univ, Lab Chem Biol & Microbial Pathogenesis, New York, NY 10065 USA

来源：

BIOORGANIC & MEDICINAL CHEMISTRY LETTERS | 2008年 / 18卷 / 22期

关键词：

Click chemistry; Chemical probe; Fatty acid; Mechanism-based probe; Activity-based probe; Bioorthogonal labeling;

D O I：

10.1016/j.bmcl.2008.09.083

中图分类号：

R914 [药物化学];

学科分类号：

100701 ;

摘要：

Chemical probes appended with reactive electrophiles afford powerful tools for pro. ling discrete protein families in living cells. Herein, we have synthesized cell-permeable chemical probes that target fatty acid-associated proteins. These fatty acid-based chemical probes contain acyloxymethylketone or fluorophosphonate functional groups and an alkyne click chemistry tag for visualization of covalently modified proteins by in-gel fluorescence scanning. Our fatty acid-based chemical probe affords new tools to evaluate the activity/expression of lipid-associated proteins that should facilitate their functional characterization and inhibitor discovery. Published by Elsevier Ltd.

引用

页码：5982 / 5986

页数：5

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