TRANSIENT EXPRESSION OF ARTIFICIAL microRNAs CONFERS RESISTANCE TO GRAPEVINE VIRUS A IN NICOTIANA BENTHAMIANA

被引：0

作者：

Roumi, V. ^{[1
]}

Afsharifar, A. ^{[1
]}

Saldarelli, P. ^{[2
]}

Niazi, A. ^{[3
]}

Martelli, G. P. ^{[4
]}

Izadpanah, K. ^{[1
]}

机构：

[1] Shiraz Univ, Coll Agr, Plant Virol Res Ctr, Shiraz, Iran

[2] UOS Ban, CNR, Ist Virol Vegetale, I-70126 Bari, Italy

[3] Shiraz Univ, Inst Biotechnol, Shiraz, Iran

[4] Univ Aldo Moro, I-70126 Bari, Italy

来源：

JOURNAL OF PLANT PATHOLOGY | 2012年 / 94卷 / 03期

关键词：

artificial microRNA; GVA; silencing; resistance; Nicotiana benthamiana; COAT PROTEIN; GENE; IDENTIFICATION; SUPPRESSION; GENOME;

D O I：

暂无

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

MicroRNAs are post-transcriptional regulators of eukaryotic organisms that function through mRNA degradation or suppression of translation. Artificial microRNA (amiRNA) act in similar way and this technology has recently attracted much attention as a tool against plant virus infections. In this study, a construct assembled on the Vitis vinifera miR166f (vvi-miR166f) pre-miRNA was used to construct an amiRNA cassette by replacing the 21 nucleotides (nt) of the miR166f sequence with a 21 nt sequence from the Grapevine virus A (GVA) genome. Two amiRNAs cassettes were engineered with 21 nt sequences targeting GVA ORF1 and ORF5. Transient expression of these amiRNAs in Nicotiana benthamiana led to various levels of resistance indicating amiRNA technology as a potential tool for GVA control.

引用

页码：643 / 649

页数：7

共 50 条

[21] Transient Expression of Flavivirus Structural Proteins in Nicotiana benthamiana
Asghar, Naveed
Melik, Wessam
Paulsen, Katrine M.
Pedersen, Bendikte N.
Bo-Granquist, Erik G.
Vikse, Rose
Stuen, Snorre
Andersson, Soren
Strid, Ake
Andreassen, Ashild K.
Johansson, Magnus
VACCINES, 2022, 10 (10)
[22] Grapevine fanleaf virus (GFLV)-specific antibodies confer GFLV and Arabis mosaic virus (ArMV) resistance in Nicotiana benthamiana
Noelke, Greta
Cobanov, Pascal
Uhde-Holzem, Kerstin
Reustle, Goetz
Fischer, Rainer
Schillberg, Stefan
MOLECULAR PLANT PATHOLOGY, 2009, 10 (01) : 41 - 49
[23] Expression of artificial microRNAs in tomato confers efficient and stable virus resistance in a cell-autonomous manner
Xiaohui Zhang
Hanxia Li
Junhong Zhang
Chanjuan Zhang
Pengjuan Gong
Khurram Ziaf
Fangming Xiao
Zhibiao Ye
Transgenic Research, 2011, 20 : 569 - 581
[24] Expression of artificial microRNAs in tomato confers efficient and stable virus resistance in a cell-autonomous manner
Zhang, Xiaohui
Li, Hanxia
Zhang, Junhong
Zhang, Chanjuan
Gong, Pengjuan
Ziaf, Khurram
Xiao, Fangming
Ye, Zhibiao
TRANSGENIC RESEARCH, 2011, 20 (03) : 569 - 581
[25] Grapevine virus A-mediated gene silencing in Nicotiana benthamiana and Vitis vinifera
Muruganantham, Mookkan
Moskovitz, Yoni
Haviv, Sabrina
Horesh, Tamar
Fenigstein, Annie
du Preez, Jacques
Stephan, Dirk
Burger, Johan T.
Mawassi, Munir
JOURNAL OF VIROLOGICAL METHODS, 2009, 155 (02) : 167 - 174
[26] Infection and Colonization of Nicotiana benthamiana by Grapevine leafroll-associated virus 3
Prator, Cecilia A.
Kashiwagi, Chloe M.
Voncina, Darko
Almeida, Rodrigo P. P.
VIROLOGY, 2017, 510 : 60 - 66
[27] Optimal systemic grapevine fanleaf virus infection in Nicotiana benthamiana following agroinoculation
Osterbaan, Larissa J.
Schmitt-Keichinger, Corinne
Vigne, Emmanuelle
Fuchs, Marc
JOURNAL OF VIROLOGICAL METHODS, 2018, 257 : 16 - 21
[28] USE OF INVITRO CULTURES OF NICOTIANA-BENTHAMIANA FOR THE PURIFICATION OF GRAPEVINE VIRUS-A
MONETTE, PL
JAMES, D
PLANT CELL TISSUE AND ORGAN CULTURE, 1990, 23 (02) : 131 - 134
[29] Production of Bioactive Recombinant Reteplase by Virus-Based Transient Expression System in Nicotiana benthamiana
Ma, Ting
Li, Zhiying
Wang, Sheng
FRONTIERS IN PLANT SCIENCE, 2019, 10
[30] Artificial microRNA-mediated resistance to cucumber green mottle mosaic virus in Nicotiana benthamiana
Chaoqiong Liang
Jianjun Hao
Jianqiang Li
Barbara Baker
Laixin Luo
Planta, 2019, 250 : 1591 - 1601

← 1 2 3 4 5 →