Characterization of a novel RNA virus from Nesidiocoris tenuis related to members of the genus Iflavirus

被引:4
|
作者
Dong, Yonghao [1 ,2 ]
Chao, Jiangtao [1 ]
Liu, Jinyan [1 ]
Rice, Annabel [3 ]
Holdbrook, Robert [3 ]
Liu, Yongjie [2 ]
Xu, Pengjun [1 ,3 ]
机构
[1] Chinese Acad Agr Sci, Tobacco Res Inst, Qingdao 266101, Peoples R China
[2] Shandong Agr Univ, Coll Plant Protect, Tai An, Shandong, Peoples R China
[3] Univ Lancaster, Lancaster Environm Ctr, Lancaster, England
基金
中国国家自然科学基金;
关键词
BEE PARALYSIS VIRUS; BROWN PLANTHOPPER; GENOME SEQUENCE; TRANSMISSION;
D O I
10.1007/s00705-017-3622-8
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
The complete genome of a novel virus from Nesidiocoris tenuis was determined by RNA-seq and rapid amplification of cDNA ends. This virus has a single-stranded RNA genome of 10633 nucleotides (nt) in length, not including the poly(A) tail, and contains two putative open reading frames (ORFs). ORF1 encodes a polypeptide of 1320 amino acids (aa) with a predicted molecular mass of 147.92 kDa and theoretical isoelectric point (pI) of 6.96. ORF2 encodes a polypeptide of 1728 aa with a predicted molecular mass of 197.09 kDa and pI of 6.73. Phylogenetic analysis with the deduced aa sequences of the conserved RNA dependent RNA polymerase domain as well as whole genome nt sequences indicated that the virus clusters with viruses classified within the genus Iflavirus, with a high bootstrap value in the maximum-likelihood and neighbor-joining trees. However, this virus has a distinct genome structure with two ORFs, iflaviruses normally having one, suggesting the virus might be a prototype of a new genus. We named the virus isolate Nesidiocoris tenuis virus 1 (NtV-1). The prevalence of NtV-1 infection in wild samples of N. tenuis was at a low level (7.32%, 6 positive in 82 samples), suggesting a possible harmful effect to its host.
引用
收藏
页码:571 / 574
页数:4
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