Characterization of a novel RNA virus from the phytopathogenic fungus Leptosphaeria biglobosa related to members of the genus Mitovirus

被引:2
|
作者
Wang, Yong [1 ,2 ]
Xu, Zhiyong [1 ,2 ]
Cheng, Jiasen [1 ,2 ]
Fu, Yanping [1 ]
Jiang, Daohong [1 ,2 ]
Xie, Jiatao [1 ,2 ,3 ]
机构
[1] Huazhong Agr Univ, State Key Lab Agr Microbiol, Wuhan 430070, Hubei, Peoples R China
[2] Huazhong Agr Univ, Coll Plant Sci & Technol, Prov Key Lab Plant Pathol Hubei Prov, Wuhan 430070, Hubei, Peoples R China
[3] Huazhong Agr Univ, Coll Plant Sci & Technol, Plant Pathol Dept, Wuhan 430070, Hubei, Peoples R China
关键词
PHOMA STEM CANKER; CONFERS HYPOVIRULENCE; BRASSICA-NAPUS; MACULANS; MYCOVIRUS;
D O I
10.1007/s00705-018-04143-3
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
Mycovirus infection is a universal phenomenon in the major fungus groups. So far, however, mycoviruses have not been described in the phytopathogenic fungus Leptosphaeria biglobosa, the causal agent of phoma stem canker (blackleg). Here, we report the complete genome sequence of a novel mitovirus, Leptosphaeria biglobosa mitovirus 1 (LbMV1), isolated from the strain H3-38 of L. biglobosa. The LbMV1 genome comprises 2568 nucleotides with a low G+C content of 30%. Using the mitochondrial genetic code, the genome of LbMV1 was found to contain a single large open reading frame that encodes a predicted protein of 756 amino acids. Multiple alignment of the predicted protein sequence showed the highest similarity (51% identity) to the putative RNA-dependent RNA polymerase of Ophiostoma mitovirus 4. In addition, phylogenetic analysis grouped LbMV1 within the genus Mitovirus of the family Narnaviridae. Our results suggest that the double-stranded RNA found in strain H3-38 is the replicating genome of the mitovirus LbMV1, its deletion represents the first evidence of a mycovirus infecting L. biglobosa.
引用
收藏
页码:913 / 916
页数:4
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