Extraction of valuable compounds from Arthrospira platensis using pulsed electric field treatment

被引:70
|
作者
Jaeschke, Debora Pez [1 ]
Mercali, Giovana Domeneghini [2 ]
Ferreira Marczak, Ligia Damasceno [1 ]
Mueller, Georg [3 ]
Frey, Wolfgang [3 ]
Gusbeth, Christian [3 ]
机构
[1] Univ Fed Rio Grande do Sul, Chem Engn Dept, Engenheiro Luiz Englert St, BR-90040040 Porto Alegre, RS, Brazil
[2] Univ Fed Rio Grande do Sul, Inst Food Sci & Technol, Bento Goncalves Ave 9500, BR-91501970 Porto Alegre, RS, Brazil
[3] Karlsruhe Inst Technol, Inst Pulse Power & Microwave Technol IHM, Karlsruhe, Germany
关键词
Pulsed electric field; Phycocyanin; Protein; Electroporation; Cyanobacteria; Pigments; Antioxidant capacity; BLUE-GREEN-ALGA; C-PHYCOCYANIN; ANTIOXIDANT ACTIVITY; SPIRULINA-PLATENSIS; ASSISTED EXTRACTION; PURIFICATION; PEPTIDES; GROWTH; FRUIT; ELECTROPORATION;
D O I
10.1016/j.biortech.2019.03.035
中图分类号
S2 [农业工程];
学科分类号
0828 ;
摘要
Pulsed electric field (PEF) treatment was evaluated for phycocyanin and proteins extraction from Arthrospira platensis (Spirulina platensis). PEF extractions were performed using different specific energies (28, 56 and 122 J center dot ml(-1) of suspension) and the results were compared to the extraction with bead milling. At highest PEFtreatment energies a damage of the cell morphology could be observed and the highest yields (up to 85.2 +/- 5.7 mg center dot g(-1) and 48.4 +/- 4.4 g center dot 100 g(-1) of phycocyanins and proteins, respectively) could be obtained at 122 and 56 J center dot ml(-1). The yields increased with incubation time after PEF-treatment. The antioxidant capacity of the extracts obtained after PEF-treatment was higher than of those obtained after bead milling. PEF treatment is a promising technology to obtain blue-green antioxidant extracts from A. platensis in an environmental friendly process.
引用
收藏
页码:207 / 212
页数:6
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