A label-free supersandwich electrogenerated chemiluminescence method for the detection of DNA methylation and assay of the methyltransferase activity

被引：53
|
作者：
Li, Yan [1 ]
Luo, Xiaoe [1 ]
Yan, Zhao [1 ]
Zheng, Jianbin [1 ]
Qi, Honglan [2 ]
机构：
[1] NW Univ Xian, Inst Analyt Sci, Key Lab Electroanalyt Chem, Xian 710069, Shaanxi, Peoples R China
[2] Shaanxi Normal Univ, Sch Chem & Chem Engn, Key Lab Analyt Chem Life Sci Shaanxi Prov, Xian 710062, Peoples R China
来源：
CHEMICAL COMMUNICATIONS | 2013年 / 49卷 / 37期
关键词：
ELECTROCHEMILUMINESCENCE IMMUNOSENSOR; COLORIMETRIC ASSAY; COMPLEX MATRICES; NANOPARTICLES; HYPERMETHYLATION; UNIVERSAL; SYSTEM;
D O I：
10.1039/c3cc00203a
中图分类号：
O6 [化学];
学科分类号：
0703 ;
摘要：
A method based on electrogenerated chemiluminescence (ECL) for detection of DNA methylation and assay of the methyltransferase activity is developed, and it is demonstrated that the label-free ECL method is capable of detecting methyltransferase with a detection limit of 3 x 10(-6) U mL(-1), using a supersandwich amplification technique.
引用
收藏
页码：3869 / 3871
页数：3
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