Detection of DNA-crosslinking agents with the alkaline comet assay

被引：0
|
作者：
Pfuhler, S
Wolf, HU
机构：
[1] Dept. of Pharmacology and Toxicology, University of Ulm, Ulm
[2] Universität Ulm, Abt. Pharmakologie und Toxikologie
来源：
ENVIRONMENTAL AND MOLECULAR MUTAGENESIS | 1996年 / 27卷 / 03期
关键词：
crosslinking agents; formaldehyde release; alkaline single cell gel electrophoresis;
D O I：
10.1002/(SICI)1098-2280(1996)27:3<196::AID-EM4>3.0.CO;2-D
中图分类号：
X [环境科学、安全科学];
学科分类号：
08 ; 0830 ;
摘要：
The single cell gel electrophoresis, or comet assay, under alkaline conditions is a sensitive, simple and rapid method for the detection of DNA damage at the individual cell level. its applicability as an indicator for the DNA crosslinking potency of a test substance was investigated in human white blood cells by combined treatment with the DNA damaging agent methyl methanesulphonate (MMS) for 2 hr at 37 degrees C. The known crosslinking agents cisplatinum, mitomycin C and formaldehyde, and the formaldehyde releasers diazolidinyl urea and dimethylol urea, were shown to reduce MMS-induced DNA migration in the comet essay in a concentration-dependent manner. Two other protocols, adding MMS to the cells before or after treatment with a crosslinking agent, were carried out and achieved similar results. The results of this study indicate that the comet assay is a useful tool for the detection of crosslinking agents. Advantages and limitations of this method compared to the alkaline elution technique are discussed. (C) 1996 Wiley-Liss, Inc.
引用
收藏
页码：196 / 201
页数：6
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