A potential role for an extracellular methanol oxidase secreted by Moniliophthora perniciosa in Witches' broom disease in cacao

被引:15
|
作者
de Oliveira, Bruno V. [1 ]
Teixeira, Gleidson S. [1 ]
Reis, Osvaldo [1 ]
Barau, Joan G. [1 ]
Teixeira, Paulo Jose P. L. [1 ]
do Rio, Maria Carolina S. [1 ,3 ]
Domingues, Romenia R. [3 ]
Meinhardt, Lyndel W. [2 ]
Paes Leme, Adriana F. [3 ]
Rincones, Johana [1 ]
Pereira, Goncalo A. G. [1 ]
机构
[1] Univ Estadual Campinas UNICAMP, Inst Biol, Lab Genom & Expressao, Dept Genet Evolucao & Bioagentes, BR-13083970 Campinas, SP, Brazil
[2] USDA ARS, Sustainable Perennial Crops Lab, Beltsville, MD 20705 USA
[3] CNPEM, Lab Nacl Biociencias LNBio, BR-13083970 Campinas, SP, Brazil
基金
巴西圣保罗研究基金会;
关键词
Moniliophthora perniciosa; Cacao; Witches' broom disease; Methanol oxidase; Extracellular; Pectin methylesterase; CRINIPELLIS-PERNICIOSA; ALCOHOL OXIDASE; HANSENULA-POLYMORPHA; CAUSAL AGENT; PECTIN UTILIZATION; GENE-EXPRESSION; POSTIA-PLACENTA; BIOTROPHIC-LIKE; COCOA; PROTEINS;
D O I
10.1016/j.fgb.2012.09.001
中图分类号
Q3 [遗传学];
学科分类号
071007 ; 090102 ;
摘要
The hemibiotrophic basidiomycete fungus Moniliophthora perniciosa, the causal agent of Witches' broom disease (WBD) in cacao, is able to grow on methanol as the sole carbon source. In plants, one of the main sources of methanol is the pectin present in the structure of cell walls. Pectin is composed of highly methylesterified chains of galacturonic acid. The hydrolysis between the methyl radicals and galacturonic acid in esterified pectin, mediated by a pectin methylesterase (PME), releases methanol, which may be decomposed by a methanol oxidase (MOX). The analysis of the M. pernciosa genome revealed putative max and pme genes. Real-time quantitative RT-PCR performed with RNA from mycelia grown in the presence of methanol or pectin as the sole carbon source and with RNA from infected cacao seedlings in different stages of the progression of WBD indicate that the two genes are coregulated, suggesting that the fungus may be metabolizing the methanol released from pectin. Moreover, immunolocalization of homogalacturonan, the main pectic domain that constitutes the primary cell wall matrix, shows a reduction in the level of pectin methyl esterification in infected cacao seedlings. Although MOX has been classically classified as a peroxisomal enzyme, M. perniciosa presents an extracellular methanol oxidase. Its activity was detected in the fungus culture supernatants, and mass spectrometry analysis indicated the presence of this enzyme in the fungus secretome. Because M. pernciosa possesses all genes classically related to methanol metabolism, we propose a peroxisome-independent model for the utilization of methanol by this fungus, which begins with the extracellular oxidation of methanol derived from the demethylation of pectin and finishes in the cytosol. (C) 2012 Elsevier Inc. All rights reserved.
引用
收藏
页码:922 / 932
页数:11
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