Degradable Segmented Polyurethane Elastomers for Bone Tissue Engineering: Effect of Polycaprolactone Content

被引:9
|
作者
Kavlock, Katherine D. [1 ]
Whang, Kyumin [2 ]
Guelcher, Scott A. [3 ]
Goldstein, Aaron S. [1 ,4 ]
机构
[1] Virginia Polytech Inst & State Univ, Sch Biomed Engn & Sci, Blacksburg, VA 24061 USA
[2] Univ Texas Hlth Sci Ctr San Antonio, Dept Restorat Dent, San Antonio, TX 78229 USA
[3] Vanderbilt Univ, Dept Chem Engn, Nashville, TN 37235 USA
[4] Virginia Polytech Inst & State Univ, Dept Chem Engn, Blacksburg, VA 24061 USA
基金
美国国家卫生研究院;
关键词
Polyurethane; modulus; polycaprolactone; crystallinity; mesenchymal stem cell; osteoblastic differentiation; SELF-ASSEMBLED MONOLAYERS; MARROW STROMAL CELLS; OSTEOBLAST-LIKE CELLS; IN-VITRO; MECHANICAL-PROPERTIES; EXTRACELLULAR-MATRIX; SURFACE CHEMISTRIES; MC3T3-E1; CELLS; GROWTH-FACTORS; DIFFERENTIATION;
D O I
10.1163/156856212X624985
中图分类号
R318 [生物医学工程];
学科分类号
0831 ;
摘要
Segmented polyurethanes (PURs), consisting of degradable poly(a-hydroxy ester) soft segments and amino-acid-derived chain extenders, are biocompatible elastomers with tunable mechanical and degradative properties suitable for a variety of tissue-engineering applications. In this study, a family of linear PURs synthesized from poly(epsilon-caprolactone) (PCL) diol, 1,4-diisocyanobutane and tyramine with theoretical PCL contents of 65-80 wt% were processed into porous foam scaffolds and evaluated for their ability to support osteoblastic differentiation in vitro. Differential scanning calorimetry and mechanical testing of the foams indicated increasing polymer crystallinity and compressive modulus with increasing PCL content. Next, bone marrow stromal cells (BMSCs) were seeded into PUR scaffolds, as well as poly(lactic-co-glycolic acid) (PLGA) scaffolds, and maintained under osteogenic conditions for 14 and 21 days. Analysis of cell number indicated a systematic decrease in cell density with increasing PUR stiffness at both 14 and 21 days in culture. However, at these same time points the relative mRNA expression for the bone-specific proteins osteocalcin and the growth factors bone morphogenetic protein-2 and vascular endothelial growth factor gene expression were similar among the PURs. Finally, prostaglandin E-2 production, alkaline phosphatase activity and osteopontin mRNA expression were highly elevated on the most-crystalline PUR scaffold as compared to the PLGA and PUR scaffolds. These results suggest that both the modulus and crystallinity of the PUR scaffolds influence cell proliferation and the expression of osteoblastic proteins. (C) Koninklijke Brill NV, Leiden, 2012
引用
收藏
页码:77 / 93
页数:17
相关论文
共 50 条
  • [11] Electropsun Polycaprolactone Fibres in Bone Tissue Engineering: A Review
    Nadeem Siddiqui
    Braja Kishori
    Saranya Rao
    Mohammad Anjum
    Venkata Hemanth
    Swati Das
    Esmaiel Jabbari
    Molecular Biotechnology, 2021, 63 : 363 - 388
  • [12] A novel Polycaprolactone/Hydroxyapatite scaffold for bone tissue engineering
    Song, Ho-Hyun
    Yoo, Mi-Kyong
    Moon, Hyun-Seuk
    Choi, Yun-Jaie
    Lee, Hyun-Chul
    Cho, Chong-Su
    ASBM7: ADVANCED BIOMATERIALS VII, 2007, 342-343 : 265 - +
  • [13] Baghdadite reinforced polycaprolactone scaffold for bone tissue engineering
    Bagheri, Azadeh
    Khodaei, Mohammad
    IRANIAN POLYMER JOURNAL, 2024, 33 (05) : 619 - 628
  • [14] A Comparison of Degradable Hydrogels for Endochondral Bone Tissue Engineering
    Daly, A. C.
    Cunniffe, G.
    Jeon, O.
    Alsberg, E.
    Kelly, D. J.
    TISSUE ENGINEERING PART A, 2015, 21 : S196 - S196
  • [15] Simvastatin-loaded graphene oxide embedded in polycaprolactone-polyurethane nanofibers for bone tissue engineering applications
    Rezaei, Hessam
    Shahrezaee, Mostafa
    Monfared, Marziyeh Jalali
    Karkan, Sonia Fathi
    Ghafelehbashi, Robabehbeygom
    JOURNAL OF POLYMER ENGINEERING, 2021, 41 (05) : 375 - 386
  • [16] Hydroxyapatite/Polyurethane Scaffolds for Bone Tissue Engineering
    Zhang, Tianyu
    Li, Jingxuan
    Wang, Yahui
    Han, Weimo
    Wei, Yan
    Hu, Yinchun
    Liang, Ziwei
    Lian, Xiaojie
    Huang, Di
    TISSUE ENGINEERING PART B-REVIEWS, 2024, 30 (01) : 60 - 73
  • [17] New building blocks for thermoplastic polyurethane elastomers for soft tissue engineering
    Potzmann, P. M.
    Seidler, K.
    Ligon, S. C.
    Koch, T.
    Stampfl, J.
    Liska, R.
    JOURNAL OF TISSUE ENGINEERING AND REGENERATIVE MEDICINE, 2012, 6 : 215 - 215
  • [18] Effect of soft segment chemistry on marine-biodegradation of segmented polyurethane elastomers
    Nguyen, Thinh Van
    An, Yingjun
    Kusano, Yasunori
    Kageoka, Masakazu
    Feng, Sinan
    Padermshoke, Adchara
    Masunaga, Hiroyasu
    Sasaki, Sono
    Takahara, Atsushi
    POLYMER DEGRADATION AND STABILITY, 2025, 233
  • [19] Nanophase separation in segmented polyurethane elastomers:: Effect of specific interactions on structure and properties
    Pukanszky, Bela
    Bagdi, Kristof
    Tovolgyi, Zsuzsa
    Varga, Jozsef
    Botz, Lajos
    Hudak, Stephan
    Doczi, Tamas
    Pukanszky, Bela
    EUROPEAN POLYMER JOURNAL, 2008, 44 (08) : 2431 - 2438
  • [20] Effect of the Addition of Inorganic Fillers on the Properties of Degradable Polymeric Blends for Bone Tissue Engineering
    Marecik, Stanislaw
    Pudelko-Prazuch, Iwona
    Balasubramanian, Mareeswari
    Ganesan, Sundara Moorthi
    Chatterjee, Suvro
    Pielichowska, Kinga
    Kandaswamy, Ravichandran
    Pamula, Elzbieta
    MOLECULES, 2024, 29 (16):