Gene detection and expression profiling of Neisseria meningitidis using NanoString nCounter platform

被引：2

作者：

Tekletsion, Yenenesh K. ^{[1
]}

Christensen, Hannah ^{[2
]}

Finn, Adam ^{[1
,2
]}

机构：

[1] Univ Bristol, Sch Cellular & Mol Med, Bristol BS8 1TD, Avon, England

[2] Univ Bristol, Bristol Med Sch, Populat Hlth Sci, Bristol BS8 2BN, Avon, England

来源：

JOURNAL OF MICROBIOLOGICAL METHODS | 2018年 / 146卷

关键词：

Transcriptomics; NanoString; Gene expression; Neisseria meningitidis; mRNA; IDENTIFICATION; MENINGOCOCCUS; COLONIZATION; CARRIAGE;

D O I：

10.1016/j.mimet.2018.02.003

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Detection of bacterial gene transcripts in low density mucosal samples is challenging. We evaluated the NanoString nCounter system for transcript detection in Neisseria meningitidis (Nm) cultures. The method was sensitive, reproducible (R-2 = 0.99) and demonstrated changes in gene expression. Studying Nm transcripts from pharyngeal samples may be feasible using this approach.

引用

页码：100 / 103

页数：4

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