Gene detection and expression profiling of Neisseria meningitidis using NanoString nCounter platform

被引：2

作者：

Tekletsion, Yenenesh K. ^{[1
]}

Christensen, Hannah ^{[2
]}

Finn, Adam ^{[1
,2
]}

机构：

[1] Univ Bristol, Sch Cellular & Mol Med, Bristol BS8 1TD, Avon, England

[2] Univ Bristol, Bristol Med Sch, Populat Hlth Sci, Bristol BS8 2BN, Avon, England

来源：

JOURNAL OF MICROBIOLOGICAL METHODS | 2018年 / 146卷

关键词：

Transcriptomics; NanoString; Gene expression; Neisseria meningitidis; mRNA; IDENTIFICATION; MENINGOCOCCUS; COLONIZATION; CARRIAGE;

D O I：

10.1016/j.mimet.2018.02.003

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Detection of bacterial gene transcripts in low density mucosal samples is challenging. We evaluated the NanoString nCounter system for transcript detection in Neisseria meningitidis (Nm) cultures. The method was sensitive, reproducible (R-2 = 0.99) and demonstrated changes in gene expression. Studying Nm transcripts from pharyngeal samples may be feasible using this approach.

引用

页码：100 / 103

页数：4

共 50 条

[1] Colorectal cancer gene expression profiling using nanostring nCounter analysis
Ciombor, Kristen Keon
Deane, Natasha G.
Lewis, Keeli B.
Chen, Xi
Zhang, Bing
Washington, Mary Kay
Shi, Chanjuan
Beauchamp, R. Daniel
JOURNAL OF CLINICAL ONCOLOGY, 2013, 31 (15)
[2] Development and Evaluation of a Pan-Sarcoma Fusion Gene Detection Assay Using the NanoString nCounter Platform
Chang, K. T.
Goytain, A.
Tucker, T.
Lee, C.
Nielsen, T. O.
Ng, T. L.
JOURNAL OF MOLECULAR DIAGNOSTICS, 2017, 19 (06): : 1012 - 1012
[3] Derivation of a parsimonious Tuberculosis gene signature using the digital NanoString nCounter platform
Kaipilyawar, Vaishnavi
Zhao, Yue
Wang, Xutao
Joseph, Noyal M.
Babu, Senbagavalli Prakash
Hochberg, Natasha S.
Sarkar, Sonali
Horsburgh, Charles
Ellner, Jerrold J.
Johnson, William Evan
Salgame, Padmini
JOURNAL OF IMMUNOLOGY, 2022, 208 (01):
[4] Development and Evaluation of a Pan-Sarcoma Fusion Gene Detection Assay Using the NanoString nCounter Platform
Chang, Kenneth T. E.
Goytain, Angela
Tucker, Tracy
Karsan, Aly
Lee, Cheng-Han
Nielsen, Torsten O.
Ng, Tony L.
JOURNAL OF MOLECULAR DIAGNOSTICS, 2018, 20 (01): : 63 - 77
[5] A new normalization for Nanostring nCounter gene expression data
Molania, Ramyar
Gagnon-Bartsch, Johann A.
Dobrovic, Alexander
Speed, Terence P.
NUCLEIC ACIDS RESEARCH, 2019, 47 (12) : 6073 - 6083
[6] Gene Expression Profiling by Nanostring nCounter miRGE Assay May Identify Malignant Thyroid Nodules
Meunier, Danielle
Izevbaye, Iyare
LABORATORY INVESTIGATION, 2016, 96 : 151A - 151A
[7] Gene Expression Profiling by Nanostring nCounter miRGE Assay May Identify Malignant Thyroid Nodules
Meunier, Danielle
Izevbaye, Iyare
MODERN PATHOLOGY, 2016, 29 : 151A - 151A
[8] Development and Validation of a Parsimonious Tuberculosis Gene Signature Using the digital NanoString nCounter Platform
Kaipilyawar, Vaishnavi
Zhao, Yue
Wang, Xutao
Joseph, Noyal M.
Knudsen, Selby
Babu, Senbagavalli Prakash
Muthaiah, Muthuraj
Hochberg, Natasha S.
Sarkar, Sonali
Horsburgh, Charles R. Jr Jr
Ellner, Jerrold J.
Johnson, W. Evan
Salgame, Padmini
CLINICAL INFECTIOUS DISEASES, 2022, 75 (06) : 1022 - 1030
[9] Gene expression profiling of anaplastic thyroid carcinoma and coexisting differentiated thyroid carcinoma using the NanoString nCounter system.
Hashimoto, Sachie
Bando, Hiroko
Noguchi, Emiko
Kondo, Yuzuru
Ueda, Aya
Terasaki, Azusa
Okazaki, Mai
Tsushima, Yukiko
Ichioka, Emika
Iguchi-Manaka, Akiko
Hara, Hisato
JOURNAL OF CLINICAL ONCOLOGY, 2021, 39 (15)
[10] Identification of gene expression signatures in human leukemia cell lines using Nanostring gene expression profiling platform
Ismail, Imilia
Johari, Syed Ahmad Tajudin Tuan
WanTaib, Wan Rohani
Zahary, Mohd Nizam
Wong, Kah Keng
Hassan, Rosline
HUMAN GENOMICS, 2018, 12

← 1 2 3 4 5 →