A study of the expression and intracellular localization of the protein TspO in the phototrophic bacterium Rhodobacter sphaeroides

被引：0
|
作者：
Yeliseev, AA
Kaplan, S
机构：
[1] Russian Acad Sci, Bach Inst Biochem, Moscow 117071, Russia
[2] Univ Texas, Hlth Sci Ctr, Houston Med Sch, Dept Microbiol & Mol Genet, Houston, TX 77225 USA
来源：
APPLIED BIOCHEMISTRY AND MICROBIOLOGY | 1999年 / 35卷 / 03期
关键词：
D O I：
暂无
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
The expression of the gene tspO, which encodes the 17-kDa protein TspO, has been studied. TspO is unusually rich in aromatic amino acids, L-tryptophan in particular. TspO of the phototrophic bacterium Rhodobacter sphaeroides is homologous to the mitochondrial benzodiazepine receptor of animals. TspO was expressed in E. coli cells, purified, and used as an antigen for obtaining polyclonal antibodies. The antibodies specifically reacted with TspO of the outer membrane fraction of R, sphaeroides. The transition of the cells from aerobic to anaerobic (photosynthetic) type of metabolism increased the level of TspO severalfold. Possible mechanisms of regulation of the physiological activity of TspO in R. sphaeroides are discussed.
引用
收藏
页码：284 / 290
页数：7
相关论文
共 50 条
  • [31] Overexpression of pucC improves the heterologous protein expression level in a Rhodobacter sphaeroides expression system
    Cheng, L.
    Chen, G.
    Ding, G.
    Zhao, Z.
    Dong, T.
    Hu, Z.
    GENETICS AND MOLECULAR RESEARCH, 2015, 14 (02) : 4058 - 4067
  • [32] ELECTROGENESIS ASSOCIATED WITH PROTON-TRANSFER IN THE REACTION CENTER PROTEIN OF THE PURPLE BACTERIUM RHODOBACTER-SPHAEROIDES
    DRACHEV, LA
    MAMEDOV, MD
    MULKIDJANIAN, AY
    SEMENOV, AY
    SHINKAREV, VP
    VERKHOVSKY, MI
    FEBS LETTERS, 1990, 259 (02) : 324 - 326
  • [33] A novel T7 RNA polymerase dependent expression system for high-level protein production in the phototrophic bacterium Rhodobacter capsulatus
    Katzke, Nadine
    Arvani, Solmaz
    Bergmann, Rene
    Circolone, Franco
    Markert, Annette
    Svensson, Vera
    Jaeger, Karl-Erich
    Heck, Achim
    Drepper, Thomas
    PROTEIN EXPRESSION AND PURIFICATION, 2010, 69 (02) : 137 - 146
  • [34] The Hfq-like protein NrfA of the phototrophic purple bacterium Rhodobacter capsulatus controls nitrogen fixation via regulation of nifA and anfA expression
    Drepper, T
    Raabe, K
    Giaourakis, D
    Gendrullis, M
    Masepohl, B
    Klipp, W
    FEMS MICROBIOLOGY LETTERS, 2002, 215 (02) : 221 - 227
  • [35] Light-mediated control of gene expression in the anoxygenic phototrophic bacterium Rhodobacter capsulatus using photocaged inducers
    Hilgers, Fabienne
    Hogenkamp, Fabian
    Klaus, Oliver
    Kruse, Luzie
    Loeschcke, Anita
    Bier, Claus
    Binder, Dennis
    Jaeger, Karl-Erich
    Pietruszka, Jörg
    Drepper, Thomas
    Frontiers in Bioengineering and Biotechnology, 2022, 10
  • [36] Light-mediated control of gene expression in the anoxygenic phototrophic bacterium Rhodobacter capsulatus using photocaged inducers
    Hilgers, Fabienne
    Hogenkamp, Fabian
    Klaus, Oliver
    Kruse, Luzie
    Loeschcke, Anita
    Bier, Claus
    Binder, Dennis
    Jaeger, Karl-Erich
    Pietruszka, Joerg
    Drepper, Thomas
    FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY, 2022, 10
  • [37] EFFECT OF AEROBIC GROWTH-CONDITIONS ON THE SOLUBLE CYTOCHROME CONTENT OF THE PURPLE PHOTOTROPHIC BACTERIUM RHODOBACTER-SPHAEROIDES - INDUCTION OF CYTOCHROME-C554
    BARTSCH, RG
    AMBLER, RP
    MEYER, TE
    CUSANOVICH, MA
    ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS, 1989, 271 (02) : 433 - 440
  • [38] SPONTANEOUS AND REVERSIBLE HIGH-FREQUENCY FRAMESHIFTS ORIGINATING A PHASE-TRANSITION IN THE CAROTENOID BIOSYNTHESIS PATHWAY OF THE PHOTOTROPHIC BACTERIUM RHODOBACTER-SPHAEROIDES 2.4.1
    GARI, E
    GIBERT, I
    BARBE, J
    MOLECULAR & GENERAL GENETICS, 1992, 232 (01): : 74 - 80
  • [39] Involvement in denitrification of the napKEFDABC genes encoding the periplasmic nitrate reductase system in the denitrifying phototrophic bacterium Rhodobacter sphaeroides f. sp. denitrificans
    Liu, HP
    Takio, S
    Satoh, T
    Yamamoto, I
    BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY, 1999, 63 (03) : 530 - 536
  • [40] ???????Nickel ion extracellular uptake by the phototrophic bacterium Rhodobacter sphaeroides: new insights from Langmuir modelling and X-ray photoelectron spectroscopic analysis
    Chirizzi, Daniela
    Mastrogiacomo, Disma
    Semeraro, Paola
    Milano, Francesco
    De Bartolomeo, Anna Rita
    Trotta, Massimo
    Valli, Ludovico
    Giotta, Livia
    Guascito, Maria Rachele
    APPLIED SURFACE SCIENCE, 2022, 593
12345