A study of the expression and intracellular localization of the protein TspO in the phototrophic bacterium Rhodobacter sphaeroides

被引：0
|
作者：
Yeliseev, AA
Kaplan, S
机构：
[1] Russian Acad Sci, Bach Inst Biochem, Moscow 117071, Russia
[2] Univ Texas, Hlth Sci Ctr, Houston Med Sch, Dept Microbiol & Mol Genet, Houston, TX 77225 USA
来源：
APPLIED BIOCHEMISTRY AND MICROBIOLOGY | 1999年 / 35卷 / 03期
关键词：
D O I：
暂无
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
The expression of the gene tspO, which encodes the 17-kDa protein TspO, has been studied. TspO is unusually rich in aromatic amino acids, L-tryptophan in particular. TspO of the phototrophic bacterium Rhodobacter sphaeroides is homologous to the mitochondrial benzodiazepine receptor of animals. TspO was expressed in E. coli cells, purified, and used as an antigen for obtaining polyclonal antibodies. The antibodies specifically reacted with TspO of the outer membrane fraction of R, sphaeroides. The transition of the cells from aerobic to anaerobic (photosynthetic) type of metabolism increased the level of TspO severalfold. Possible mechanisms of regulation of the physiological activity of TspO in R. sphaeroides are discussed.
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页码：284 / 290
页数：7
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