ARF6, PI3-kinase and host cell actin cytoskeleton in Toxoplasma gondii cell invasion

被引：26

作者：

da Silva, Claudio Vieira ^{[1
,3
]}

da Silva, Erika Alves ^{[1
]}

Cruz, Mario Costa ^{[1
]}

Chavrier, Philippe ^{[2
]}

Mortara, Renato Arruda ^{[1
]}

机构：

[1] Univ Fed Sao Paulo, Dept Microbiol Imunol & Parasitol, BR-04023062 Sao Paulo, Brazil

[2] Inst Curie, CNRS, UMR144, F-75248 Paris, France

[3] Univ Fed Uberlandia, Inst Ciencias Biomed, BR-38400902 Uberlandia, MG, Brazil

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 2009年 / 378卷 / 03期

基金：

巴西圣保罗研究基金会;

关键词：

Toxoplasma gondii; Host cell invasion; Parasitophorous vacuole; ARF6; PI3-kinase; Actin cytoskeleton; PLASMA-MEMBRANE; BACTERIAL INVASION; PROTEIN; PHAGOCYTOSIS; PENETRATION; MACROPHAGES; 3-KINASE; VACUOLE; KINASE; PHOSPHOINOSITIDES;

D O I：

10.1016/j.bbrc.2008.11.108

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Toxoplasma gondii infects a variety of different cell types in a range of different hosts. Host cell invasion by T. gondii occurs by active penetration of the host cell, a process previously described as independent of host actin polymerization. Also, the parasitophorous vacuole has been shown to resist fusion with endocytic and exocytic pathways of the host cell. ADP-ribosylation factor-6 (ARF6) belongs to the ARE family of small GTP-binding proteins. ARF6 regulates membrane trafficking and actin cytoskeleton rearrangements at the plasma membrane. Here, we have observed that ARF6 is recruited to the parasitophorous vacuole of tachyzoites of T. gondii RH strain and it also plays an important role in the parasite cell invasion with activation of PI3-kinase and recruitment of PIP2 and PIP3 to the parasitophorous vacuole of invading parasites. Moreover, it was verified that maintenance of host cell actin cytoskeleton integrity is important to parasite invasion. (C) 2008 Elsevier Inc. All rights reserved.

引用

页码：656 / 661

页数：6

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