Cloning and expression profiles of yak lipoprotein lipase gene

被引：4

作者：

Ding, Yi ^{[1
]}

Xu, Yaou ^{[1
]}

Lin, Yaqiu ^{[1
]}

Yue, Yu ^{[2
]}

Jin, Suyu ^{[1
]}

Li, Yuping ^{[3
]}

Zheng, Yucai ^{[1
]}

机构：

[1] SW Univ Nationalities, Coll Life Sci & Technol, Chengdu 610041, Peoples R China

[2] Sci & Technol Bur Ganzi Prefecture, Kangding 626000, Sichuan, Peoples R China

[3] Agr & Anim Husb Bur Jiulong Cty, Jiulong 626200, Sichuan, Peoples R China

来源：

JOURNAL OF APPLIED ANIMAL RESEARCH | 2012年 / 40卷 / 04期

关键词：

Bos grunniens; lipoprotein lipase; muscle; fat metabolism; gene expression; SKELETAL-MUSCLE; TISSUES; RATS;

D O I：

10.1080/09712119.2012.692325

中图分类号：

S8 [畜牧、动物医学、狩猎、蚕、蜂];

学科分类号：

0905 ;

摘要：

The objective of this study was to reveal the sequence characteristic and expression pattern of lipoprotein lipase (LPL) gene in high altitude yaks (Bos grunniens). A full-length cDNA of LPL was cloned from yak liver by RT-PCR. The cDNA obtained was 1591 nucleotide (nt) long with a 1437 nt open reading frame encoding 478 amino acids. Yak LPL shares 99.5% nucleotide sequence similarity and 100% amino acid sequence similarity with cattle. Quantitative real-time PCR analysis showed that yak heart and adipose tissue contained significantly higher LPL mRNA level than other tissues assayed. Yak calves contained lower level of LPL mRNA in longissimus dorsi than adult yaks (p<0.01). Besides, LPL mRNA level in longissimus dorsi was markedly lower in adult yaks that in adult cattle living at low altitude region.

引用

页码：311 / 315

页数：5

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