New mRNA isoform of Oct-1 transcription factor is transcribed from alternative promoter

被引：8

作者：

Krylova, I. D. ^{[1
,2
]}

Portseva, T. N. ^{[1
]}

Georgieva, S. G. ^{[1
]}

Stepchenko, A. G. ^{[1
]}

Pankratova, E. V. ^{[1
]}

机构：

[1] Russian Acad Sci, VA Engelhardt Mol Biol Inst, Moscow 119991, Russia

[2] Moscow State Pedag Univ, Moscow 119991, Russia

来源：

MOLECULAR BIOLOGY | 2013年 / 47卷 / 04期

关键词：

gene expression; Oct-1; alternative promoters; EXPRESSION; COMPLEX; SITES;

D O I：

10.1134/S0026893313040080

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The Oct-1 transcription factor belongs to a family of highly conserved POU domain proteins that participate in the regulation of housekeeping and tissue-specific gene expression. Several isoforms of Oct-1 were described previously. We have found the new alternative promoter of Oct-1 gene and the corresponding mRNA Oct-1X. This mRNA encodes the alternative Oct-1 isoform, which has a truncated N-terminus compared to Oct-1A or Oct-1L isoforms. We explored the expression pattern of this isoform in human tissues and cell lines and demonstrated that it is ubiquitously expressed, but its expression strongly varies in different tissues. Using cotransfection and double luciferase assay, we have demonstrated that Oct-1X is the activator of housekeeping (histone H2B) and tissue-specific genes (B29) transcription.

引用

页码：552 / 558

页数：7

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