HPLC-UV Determination of Dextromethorphan in Syrup Method validation

被引：0
|
作者：
Avram, Nicolae [1 ]
Heghes, Simona Codruta [2 ]
Rus, Luca-Liviu [3 ]
Juncan, Anca Maria [3 ]
Rus, Lucia Maria [2 ]
Filip, Lorena [4 ]
Roman-Filip, Corina [5 ,6 ]
机构：
[1] SC Bioef SRL, Independent Drug Anal Lab, 1 Ferma Str, Dostat 517275, Alba, Romania
[2] Iuliu Hatieganu Univ Med & Pharm, Dept Drug Anal, 6 Louis Pasteur Str, Cluj Napoca 400349, Romania
[3] Lucian Blaga Univ, Fac Med, Preclin Dept, 2 A Lucian Blaga Str, Sibiu 550169, Romania
[4] Iuliu Hatieganu Univ Med & Pharm, Dept Bromatol Hyg Nutr, 8 Victor Babes Str, Cluj Napoca 400012, Romania
[5] Lucian Blaga Univ, Fac Med, Clin Dept, 2 A Lucian Blaga Str, Sibiu 550169, Romania
[6] Acad Emergency Hosp Sibiu, Dept Neurol, 2-4 Pompeiu Onofreiu Str, Sibiu 550166, Romania
来源：
REVISTA DE CHIMIE | 2019年 / 70卷 / 02期
关键词：
dextromethorphan; HPLC; validation; syrup; LIQUID-CHROMATOGRAPHIC DETERMINATION; PSEUDOEPHEDRINE HYDROCHLORIDE; SODIUM BENZOATE; COLD MEDICINES; COUGH; HYDROBROMIDE; GUAIFENESIN; CHLORPHENIRAMINE; DIPHENHYDRAMINE; PHARMACOLOGY;
D O I：
暂无
中图分类号：
O6 [化学];
学科分类号：
0703 ;
摘要：
A HPLC-UV method, for determination of dextromethorphan hydrobromide in syrup, was validated. The chromatographic analysis was performed using an RP-18, Nucleodur chromatographic column (250 mm x 4 mm, 5 pm) at constant temperature (50 degrees C) with a mobile phase consisting of a mixture of acetonitrile/methanol (70:30 v/v) with sodium docusate (as ion pair agent) and ammonium nitrate, pH = 3.4. The flow rate of the mobile phase was 1 mL/min and the detection was carried out at 280 nm. System suitability, specificity, linearity, precision, accuracy, limit of detection and limit of quantification agreed with current pharmacopeial requests. The method is suitable for routine analysis of dextromethorphan hydrobromide in syrup.
引用
收藏
页码：487 / 490
页数：4
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