Simultaneous measurements of a specimen quantitative-phase signal and its surrounding medium refractive index using quantitative-phase imaging

被引：3
|
作者：
de Dorlodot, Bertrand [2 ]
Belanger, Erik [2 ,3 ,4 ]
Rioux-Pellerin, Emile [2 ]
Marquet, Pierre [1 ,2 ,4 ]
机构：
[1] Univ Laval, Dept Psychiat & Neurosci, Quebec City, PQ G1V 0A6, Canada
[2] Univ Laval, Ctr Rech CERVO, Quebec City, PQ G1J 2G3, Canada
[3] Univ Laval, Dept Phys Genie Phys & Opt, Quebec City, PQ G1V 0A6, Canada
[4] Univ Laval, Ctr Opt Photon & Laser, Quebec City, PQ G1V 0A6, Canada
来源：
OPTICS LETTERS | 2020年 / 45卷 / 19期
基金：
加拿大创新基金会; 加拿大自然科学与工程研究理事会;
关键词：
DIGITAL HOLOGRAPHIC MICROSCOPY; CELLS;
D O I：
10.1364/OL.391641
中图分类号：
O43 [光学];
学科分类号：
070207 ; 0803 ;
摘要：
This Letter demonstrates a method to simultaneously measure the quantitative-phase signal (QPS) of the observed. specimen and the refractive index of its surrounding medium (n(m)) in a time-resolved manner using a micro-structured coverslip. Such coverslips, easily integrated into perfused live-cell imaging chambers, allow to use various quantitative-phase imaging techniques to achieve this dual measurement. Since QPS is crucially dependent on n(m), the measurement of the latter paves the way for its manipulation in a controlled manner leading to a QPS contrast modulation for appealing applications, including visualizing the interior of cells. (C) 2020 Optical Society of America
引用
收藏
页码：5587 / 5590
页数：4
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