Development of an ultra-sensitive enzyme immunoassay for human insulin autoantibodies

被引：5
|
作者：
Numata, Satoshi [2 ]
Umehara, Asako [3 ]
Katakami, Hideki [4 ]
Inoue, Shinobu [5 ]
Hashida, Seiichi [1 ]
机构：
[1] Tokushima Bunri Univ, Inst Hlth Sci, Tokushima 7708514, Japan
[2] Kochi Univ, Dept Hlth Sci, Kochi 7818515, Japan
[3] Takamatsu Red Cross Hosp, Takamatsu, Kagawa 7600017, Japan
[4] Teikyo Univ, Dept Med, Chiba Med Ctr, Chiba 2990111, Japan
[5] Miyazaki Higashi Hosp, Natl Hosp Org, Miyazaki 8800911, Japan
来源：
CLINICAL BIOCHEMISTRY | 2012年 / 45卷 / 13-14期
关键词：
Enzyme immunoassay; Insulin antibodies; Diabetes; Graves disease; Dextran-charcoal; VIRUS TYPE-1 P24; ANTIBODIES; SERUM; ADULTS; LADA; IGG;
D O I：
10.1016/j.clinbiochem.2012.05.027
中图分类号：
R446 [实验室诊断]; R-33 [实验医学、医学实验];
学科分类号：
1001 ;
摘要：
Objectives: We developed an ultrasensitive enzyme immunoassay (ICT-EIA) for insulin autoantibody (IAA) measurements to better understand the pathophysiology of diabetes. Design and methods: We developed ICT-EIA for IAA and measured IAA in 24 patients with type 1 diabetes, 30 patients with type 2 diabetes. 30 patients with methimazole-treated Graves' disease, 20 patients with Hashimoto's disease, 9 patients with hyperinsulinemia, and 73 healthy control subjects. Results: The conventional ELISA identified 3 patients with type 1 diabetes and 2 patients with type 2 diabetes as IAA positive, whereas 15 patients with type 1 diabetes, 7 patients with type 2 diabetes, and 4 patients with methimazole-treated Graves' disease were identified as IAA positive using ICT-EIA. Conclusions: The ICT-EIA is an ultrasensitive and specific assay for IAA, and its use may provide a better understanding of the role of IAA in diabetes onset and progression. (c) 2012 The Canadian Society of Clinical Chemists. Published by Elsevier Inc. All rights reserved.
引用
收藏
页码：1086 / 1091
页数：6
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