The aim of this study was to determine sprint profiles of professional female soccer players and evaluate how various speed thresholds impact those outcomes. Seventy-one professional players competing in full matches were assessed repeatedly during 12 regular season matches using a Global Positioning System (GPS). Locomotion >= 18 km.h(-1) was defined as sprinting and each event was classified into: Zone 1: 18.0-20.9 km.h(-1); Zone 2: 21.0-22.9 km.h(-1); Zone 3: 23.024.9 km.h(-1) and Zone 4: 425 km.h(-1). Outcomes included: duration (s), distance (m), maximum speed (km.h(-1)), duration since previous sprint (min) and proportion of total sprint distance. In total 5,019 events were analysed from 139 player-matches. Mean sprint duration, distance, maximum speed and time between sprints were 2.3 +/- 1.5 s, 15.1 +/- 9.4 m, 21.8 +/- 2.3 km.h(-1), and 2.5 +/- 2.5 min, respectively. Mean sprint distances were 657 +/- 157, 447 +/- 185, and 545 +/- 217 m for forwards, midfielders and defenders, respectively (P < 0.046). Midfielders had shorter sprint duration (P = 0.023), distance (P <= 0.003) and maximum speed (P < 0.001), whereas forwards performed more sprints per match (43 +/- 10) than midfielders (31 +/- 11) and defenders (36 +/- 12) (P <= 0.016). Forty-five percent, 29%, 15%, and 11% of sprints occurred in sprint Zones 1, 2, 3 and 4, respectively. This group of professional female soccer players covered 5.3 +/- 2.0% of total distance >= 18 km.h(-1) with positional differences and percent decrements distinct from other previously identified elite players. These data should guide the development of high intensity and sprint thresholds for elite-standard female soccer players.