Effects of overexpression of the endogenous farnesyl diphosphate synthase on the artemisinin content in Artemisia annua L.

被引：88
|
作者：
Han, JL
Liu, BY
Ye, HC [1 ]
Wang, H
Li, ZQ
Li, GF
机构：
[1] Chinese Acad Sci, Inst Bot, Key Lab Photosynth & Environm Mol Physiol, Beijing 100093, Peoples R China
[2] Grad Univ, Chinese Acad Sci, Beijing 100039, Peoples R China
[3] Hebei Univ, Coll Life Sci, Hebei 071002, Peoples R China
来源：
JOURNAL OF INTEGRATIVE PLANT BIOLOGY | 2006年 / 48卷 / 04期
关键词：
Artemisia annua; artemisinin; artemisinin content; farnesyl diphosphate synthase; overexpression;
D O I：
10.1111/j.1744-7909.2006.00208.x
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Artemisinin is a novel effective antimalarial drug extracted from the medicinal plant Artemisia annua L. Owing to the tight market and low yield of artemisinin, there is great interest in enhancing the production of artemisinin. In the present study, farnesyl diphosphate synthase (FPS) was overexpressed in high-yield A. annua to increase the artemisinin content. The FPS activity in transgenic A. annua was two- to threefold greater than that in non-transgenic A. annua. The highest artemisinin content in transgenic A. annua was approximately 0.9% (dry weight), which was 34.4% higher than that in non-transgenic A. annua. The results demonstrate the regulatory role of FPS in artemisinin biosynthesis.
引用
收藏
页码：482 / 487
页数：6
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