Cloning and analysis of a cDNA encoding farnesyl diphosphate synthase from Artemisia annua

被引：84
|
作者：
Matsushita, Y
Kang, W
Charlwood, BV
机构：
[1] Division of Life Sciences, King's College London, London
[2] Division of Life Sciences, King's College London, London W8 7AH, Campden Hill Road
[3] Department of Biology, Imperial College, London SW7 2BB, Prince Consort Road
来源：
GENE | 1996年 / 172卷 / 02期
基金：
英国生物技术与生命科学研究理事会;
关键词：
artemisinin; cloned gene expression; EPP synthase; isoprenoid biosynthesis; medicinal plant; sesquiterpenoid;
D O I：
10.1016/0378-1119(96)00054-6
中图分类号：
Q3 [遗传学];
学科分类号：
071007 ; 090102 ;
摘要：
A cDNA encoding farnesyl diphosphate (FPP) synthase (FPPS) has been cloned from a cDNA library of Artemisia annua. The sequence analysis showed that the cDNA encoded a protein of 343 amino acid (aa) residues with a calculated molecular weight of 39 420 kDa. The deduced aa sequence of the cDNA was highly similar to FPPS from other plants, yeast and mammals, and contained the two conserved domains found in polyprenyl synthases including FPPS, geranylgeranyl diphosphate synthases and hexaprenyl diphosphate synthases. The expression of the cDNA in Escherichia coli showed enzyme activity for FPPS in vitro.
引用
收藏
页码：207 / 209
页数：3
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