Detection of maternal DNA in umbilical cord plasma by fluorescent PCR amplification of short tandem repeat sequences

被引：0

作者：

Bauer, M

Orescovic, I

Schoell, WM

Bianchi, DW

Pertl, B

机构：

[1] Graz Univ, Dept Obstet & Gynecol, A-8036 Graz, Austria

[2] Tufts Univ New England Med Ctr, Div Genet, Dept Pediat, Boston, MA 02111 USA

[3] Tufts Univ New England Med Ctr, Dept Obstet & Gynecol, Boston, MA 02111 USA

[4] Tufts Univ, Sch Med, Boston, MA 02111 USA

来源：

CIRCULATING NUCLEIC ACIDS IN PLASMA OR SERUM II | 2001年 / 945卷

关键词：

umbilical cord plasma; short tandem repeat (STR); fluorescent PCR; maternal DNA;

D O I：

暂无

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Recently, maternal DNA was detected in umbilical cord blood using PCR amplification of minisatellite sequences. The presence of maternal DNA was demonstrated in 1% to 100% of umbilical cord blood samples. The objective of this study was to determine the frequency of cord blood contamination by maternal genetic material. We used fluorescent PCR amplification of highly polymorphic short tandem repeat (STR) markers to detect maternal DNA in umbilical cord plasma.

引用

页码：161 / 163

页数：3

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