The complete mitochondrial genome of Argiope amoena is a circular molecule of 14,121bp in length, contains 13 protein-coding genes, 2 ribosomal RNAs, 21 transfer RNAs genes and a control region. The A + T content of the overall base composition of H-strand is 72.1% (T: 38.2%; C: 10.6%; A: 33.9%; G: 17.3%). ND1, ND4, ND6 and ATP6 begin with ATA as start codon, ND4L begins with ATG, ATP8, Cyt b, ND2 and ND3 genes begin with ATT, and the other two protein-coding genes begin with TTG. ATP6, ATP8, COI, COII, COIII, Cyt b, ND1, ND2, ND3, ND4L and ND5 genes are terminated with TAA as stop codon, ND6 ends with TAG, and ND4 ends with T.