cDNA macroarray for analysis of gene expression profiles in prostate cancer

被引：3
|
作者：
Zhong, WD [1 ]
He, HC
Bi, XC
Ou, RB
Jiang, SA
Liu, LS
机构：
[1] Guangzhou First Municipal Peoples Hosp, Guangzhou Med Coll, Dept Urol, Guangzhou 510180, Peoples R China
[2] Sun Yat Sen Univ, Coll Life Sci, Guangzhou 510180, Peoples R China
来源：
CHINESE MEDICAL JOURNAL | 2006年 / 119卷 / 07期
关键词：
prostate neoplasm; cDNA macroarray; gene expression profiles;
D O I：
10.1097/00029330-200604010-00009
中图分类号：
R5 [内科学];
学科分类号：
1002 ; 100201 ;
摘要：
Background Early diagnosis and timely treatment are important for improving therapeutic efficiency of prostate cancer. DNA array is a new bio-technology for disease diagnosis. This study was conducted to diagnose prostate cancer with cDNA macroarray and analysis gene expression profiles of some selective genes in prostate cancer. Methods Total RNA was isolated from patients with prostate cancer and from normal people, and poly(A) RNA was further purified. Then it was analyzed for differentially expressed genes in prostate cancer and normal prostate by cDNA macroarray system. Results There were different expressions in the nine prostate-associated specific genes in prostate cancer as compared with normal prostate, in which, 7 were significantly upregulated and 2 were down-regulated. Conclusion As a diagnostic approach at molecular level, the cDNA macroarray is an effectively diagnostic method for prostate cancer.
引用
收藏
页码：570 / 573
页数：4
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