cDNA macroarray analysis of gene expression in synoviocytes stimulated with TNFα

被引：18
|
作者：
Sugiyama, T
Ishii, S
Yamamoto, J
Irie, R
Saito, K
Otuki, T
Wakamatsu, A
Suzuki, Y
Hio, Y
Ota, T
Nishikawa, T
Sugano, S
Masuho, Y
Isogai, T
机构：
[1] Helix Res Inst, Kisarazu, Chiba 2920812, Japan
[2] Univ Tokyo, Inst Med Sci, Lab Genome Struct, Minato Ku, Tokyo 1088639, Japan
来源：
FEBS LETTERS | 2002年 / 517卷 / 1-3期
关键词：
macroarray; gene expression; tumor necrosis factor-alpha; synoviocyte; oligo-capping; rheumatoid arthritis;
D O I：
10.1016/S0014-5793(02)02588-7
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Gene expression of synoviocytes stimulated with tumor necrosis factor-alpha (TNFalpha) was studied by macroarray analysis to elucidate the cellular response and identify new biological functions of known and unknown genes. 10 035 cDNA clones were used to make cDNA macroarrays of representative genes. Synoviocytes expressed large amounts of fibronectin and collagen mRNA. Statistical analysis of the macroarray data revealed 26 genes, including six new genes, which underwent significant alteration of gene expression in response to TNFa stimulation. These findings suggest that the synoviocyte response to TNFalpha stimulation forms the basis of development of various aspects of the pathophysiology of rheumatoid arthritis. (C) 2002 Federation of European Biochemical Societies. Published by Elsevier Science B.V. All rights reserved.
引用
收藏
页码：121 / 128
页数：8
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